Journal article
Amine modification of nonporous silica nanoparticles reduces inflammatory response following intratracheal instillation in murine lungs
Toxicology letters, Vol.241, pp.207-215
01/22/2016
DOI: 10.1016/j.toxlet.2015.11.006
PMCID: PMC4691407
PMID: 26562768
Abstract
•We investigated nonporous silica NPs both bare and modified with amine functional groups (3-aminopropyltriethoxysilane (APTES)) in order to evaluate the effect of surface chemistry on biocompatibility.•No cytotoxicity was observed in a human lung cancer epithelial cell line (A549) for bare silica NPs or amine-functionalized silica NPs.•Bare silica NPs elicited a significantly higher inflammatory response compared to amine-functionalized NPs. Amorphous silica nanoparticles (NPs) possess unique material properties that make them ideal for many different applications. However, the impact of these materials on human and environmental health needs to be established. We investigated nonporous silica NPs both bare and modified with amine functional groups (3-aminopropyltriethoxysilane (APTES)) in order to evaluate the effect of surface chemistry on biocompatibility. In vitro data showed there to be little to no cytotoxicity in a human lung cancer epithelial cell line (A549) for bare silica NPs and amine-functionalized NPs using doses based on both mass concentration (below 200μg/mL) and exposed total surface area (below 14m2/L). To assess lung inflammation, C57BL/6 mice were administered bare or amine-functionalized silica NPs via intra-tracheal instillation. Two doses (0.1 and 0.5mg NPs/mouse) were tested using the in vivo model. At the higher dose used, bare silica NPs elicited a significantly higher inflammatory response, as evidence by increased neutrophils and total protein in bronchoalveolar lavage (BAL) fluid compared to amine-functionalized NPs. From this study, we conclude that functionalization of nonporous silica NPs with APTES molecules reduces murine lung inflammation and improves the overall biocompatibility of the nanomaterial.
Details
- Title: Subtitle
- Amine modification of nonporous silica nanoparticles reduces inflammatory response following intratracheal instillation in murine lungs
- Creators
- Angie S Morris - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USAAndrea Adamcakova-Dodd - Department of Occupational and Environmental Health, College of Public Health, University of Iowa, Iowa City, IA 52242, USASean E Lehman - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USAAmaraporn Wongrakpanich - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USAPeter S Thorne - Department of Occupational and Environmental Health, College of Public Health, University of Iowa, Iowa City, IA 52242, USASarah C Larsen - Department of Chemistry, University of Iowa, Iowa City, IA 52242, USAAliasger K Salem - Division of Pharmaceutics and Translational Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IA 52242, USA
- Resource Type
- Journal article
- Publication Details
- Toxicology letters, Vol.241, pp.207-215
- DOI
- 10.1016/j.toxlet.2015.11.006
- PMID
- 26562768
- PMCID
- PMC4691407
- NLM abbreviation
- Toxicol Lett
- ISSN
- 0378-4274
- eISSN
- 1879-3169
- Publisher
- Elsevier B.V
- Language
- English
- Date published
- 01/22/2016
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Civil and Environmental Engineering; Occupational and Environmental Health; Stead Family Department of Pediatrics; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Chemistry; Dental Research; Chemical and Biochemical Engineering
- Record Identifier
- 9983985853902771
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