Journal article
Amine-reactive Neutron-encoded Labels for Highly Plexed Proteomic Quantitation
Molecular & cellular proteomics, Vol.12(11), pp.3360-3369
11/2013
DOI: 10.1074/mcp.M113.032011
PMCID: PMC3820946
PMID: 23882030
Abstract
We describe a novel amine-reactive chemical label that exploits differential neutron-binding energy between
13
C and
15
N isotopes. These neutron-encoded (NeuCode) chemical labels enable up to 12-plex MS1-based protein quantification. Each structurally identical, but isotopically unique, tag is encoded with a 12.6-mDa mass difference—relative to its nearest neighbor—so that peptides bearing these NeuCode signatures do not increase spectral complexity and are detected only upon analysis with very high mass-resolving powers. We demonstrate that the method provides quantitative performance that is comparable to both metabolic labeling and isobaric tagging while combining the benefits of both strategies. Finally, we employ the tags to characterize the proteome of
Saccharomyces cerevisiae
during the diauxic shift, a metabolic transition from fermentation to aerobic respiration.
Details
- Title: Subtitle
- Amine-reactive Neutron-encoded Labels for Highly Plexed Proteomic Quantitation
- Creators
- Alexander S Hebert - From the ‡Department of Biomolecular Chemistry, University of Wisconsin, 420 Henry Mall, Madison, Wisconsin 53706Anna E Merrill - §Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, Wisconsin 53706Jonathan A Stefely - ‖Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706Derek J Bailey - §Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, Wisconsin 53706Craig D Wenger - Agilent Laboratories, Agilent Technologies, Santa Clara, California 95051Michael S Westphall - §Genome Center of Wisconsin, University of Wisconsin-Madison, 425 Henry Mall, Madison, Wisconsin 53706David J Pagliarini - ‖Department of Biochemistry, University of Wisconsin, 433 Babcock Drive, Madison, Wisconsin 53706Joshua J Coon - From the ‡Department of Biomolecular Chemistry, University of Wisconsin, 420 Henry Mall, Madison, Wisconsin 53706
- Resource Type
- Journal article
- Publication Details
- Molecular & cellular proteomics, Vol.12(11), pp.3360-3369
- DOI
- 10.1074/mcp.M113.032011
- PMID
- 23882030
- PMCID
- PMC3820946
- NLM abbreviation
- Mol Cell Proteomics
- ISSN
- 1535-9476
- eISSN
- 1535-9484
- Publisher
- The American Society for Biochemistry and Molecular Biology
- Grant note
- R01 GM080148; 5T32HG002760; 1F30AG043282 / National Institutes of Health
- Language
- English
- Date published
- 11/2013
- Academic Unit
- Pathology; Injury Prevention Research Center
- Record Identifier
- 9984047623702771
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