Journal article
Amyloid-β precursor protein expression and modulation in human embryonic stem cells: A novel role for human chorionic gonadotropin
Biochemical and biophysical research communications, Vol.364(3), pp.522-527
12/21/2007
DOI: 10.1016/j.bbrc.2007.10.021
PMID: 17959150
Abstract
The amyloid-β precursor protein (AβPP) is a ubiquitously expressed adhesion and neuritogenic protein whose processing has previously been shown to be regulated by reproductive hormones including the gonadotropin luteinizing hormone (LH) in human neuroblastoma cells. We report for the first time the expression of AβPP in human embryonic stem (hES) cells at the mRNA and protein levels. Using N- and C-terminal antibodies against AβPP, we detected both the mature and immature forms of AβPP as well as truncated variants (∼53kDa, 47kDa, and 29kDa) by immunoblot analyses. Expression of AβPP is regulated by both the stemness of the cells and pregnancy-associated hormones. Addition of human chorionic gonadotropin, the fetal equivalent of LH that is dramatically elevated during pregnancy, markedly increased the expression of all AβPP forms. These results indicate a critical molecular signaling link between the hormonal environment of pregnancy and the expression of AβPP in hES cells that is suggestive of an important function for this protein during early human embryogenesis prior to the formation of neural precursor cells.
Details
- Title: Subtitle
- Amyloid-β precursor protein expression and modulation in human embryonic stem cells: A novel role for human chorionic gonadotropin
- Creators
- Prashob Porayette - Geriatric Research Education and Clinical CenterMiguel J. Gallego - Geriatric Research Education and Clinical CenterMaria M. Kaltcheva - Geriatric Research Education and Clinical CenterSivan Vadakkadath Meethal - Geriatric Research Education and Clinical CenterCraig S. Atwood - Case Western Reserve University
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.364(3), pp.522-527
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.bbrc.2007.10.021
- PMID
- 17959150
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Language
- English
- Date published
- 12/21/2007
- Academic Unit
- Cardiology; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984354154802771
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