Journal article
An Activated Immune and Inflammatory Response Targets the Pancreas of Newborn Pigs with Cystic Fibrosis
Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], Vol.11(5), pp.506-515
2011
DOI: 10.1159/000332582
PMCID: PMC3224519
PMID: 22057257
Abstract
Background/Aims: In cystic fibrosis (CF), pancreatic disease begins in utero and progresses over time to complete destruction of the organ. Although inflammatory cells have been detected in the pancreas of humans and pigs with CF, their subtypes have not been characterized. Methods: Using four-color flow cytometry, we analyzed the surface antigens of leukocytes in pancreas, blood, and mesenteric lymph nodes (MLN) of newborn pigs with CF (CFTR-/- and CFTRΔF508/ΔF508) and in those without CF (CFTR+/-, CFTR13+/ΔF508, CFTR+/+). Pancreatic histopathology was examined with HE stain. Results: CF pig pancreas had patchy distribution of inflammatory cells with neutrophils/macrophages in dilated acini, and lymphocytes in the interstitium compared to non-CF. B cells, effector (MHC-II+) and cytotoxic (CD2+CD8+) γδ T cells, activated (MHC-II+ and/or CD25+) and effector (CD4+CD8+) αβ T helper cells, effector natural killer cells (MHC-II+CD3-CD8+), and monocytes/macrophages and neutrophils were increased in the CF pig pancreas compared to pigs without CF. Blood and MLN leukocyte populations were not different between CF and non-CF pigs. Conclusions: We discovered an activated immune response that was specific to the pancreas of newborn CF pigs; inflammation was not systemic. The presence of both innate and adaptive immune cells suggests that the disease process is complex and extensive.
Details
- Title: Subtitle
- An Activated Immune and Inflammatory Response Targets the Pancreas of Newborn Pigs with Cystic Fibrosis
- Creators
- Maisam Abu-El-Haija - Departments of Pediatrics, Iowa City, Iowa, USAMarek Sinkora - Department of Immunology and Gnotogiology, Institute of Microbiology, Academy of Sciences of the Czech Republic, v.v.i., Novy Hradek, Czech RepublicDavid K Meyerholz - Pathology, Iowa City, Iowa, USAMichael J Welsh - Internal Medicine, Iowa City, Iowa, USAPaul B McCray - Departments of Pediatrics, Iowa City, Iowa, USAJohn Butler - Microbiology, Iowa City, Iowa, USAAliye Uc - Departments of Pediatrics, Iowa City, Iowa, USA
- Resource Type
- Journal article
- Publication Details
- Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.], Vol.11(5), pp.506-515
- DOI
- 10.1159/000332582
- PMID
- 22057257
- PMCID
- PMC3224519
- NLM abbreviation
- Pancreatology
- ISSN
- 1424-3903
- eISSN
- 1424-3911
- Publisher
- Elsevier B.V
- Grant note
- DOI: 10.13039/100000002, name: National Institutes of Health, award: DK084049, HL51670, PPG HL091842; DOI: 10.13039/100000897, name: Cystic Fibrosis Foundation, award: R458-CR07; DOI: 10.13039/501100001823, name: Ministerstvo Školství, Mládeže a Tělovýchovy, award: KON-TAKT ME09089
- Language
- English
- Date published
- 2011
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Pathology; Radiation Oncology; Gastroenterology, Hepatology, Pancreatology, and Nutrition; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020795802771
Metrics
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