An Analysis of Transvection at the yellow Locus of Drosophila melanogaster

James R Morris; Ji-long Chen; Stephen T Filandrinos; Rebecca C Dunn; Ridgely Fisk; Pamela K Geyer; Chao-ting Wu

doi:10.1093/genetics/151.2.633

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An Analysis of Transvection at the yellow Locus of Drosophila melanogaster

Journal article

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An Analysis of Transvection at the yellow Locus of Drosophila melanogaster

James R Morris, Ji-long Chen, Stephen T Filandrinos, Rebecca C Dunn, Ridgely Fisk, Pamela K Geyer and Chao-ting Wu

Genetics (Austin), Vol.151(2), pp.633-651

02/01/1999

DOI: 10.1093/genetics/151.2.633

PMCID: PMC1460495

PMID: 9927457

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Abstract

Abstract Studies of a wide variety of organisms have shown that homologous sequences can exert a significant impact on each other, resulting in changes in gene sequence, gene expression, chromatin structure, and global chromosome architecture. Our work has focused on transvection, a process that can cause genes to be sensitive to the proximity of a homologue. Transvection is seen at the yellow gene of Drosophila, where it mediates numerous cases of intragenic complementation. In this article, we describe two approaches that have characterized the process of transvection at yellow. The first entailed a screen for mutations that support intragenic complementation at yellow. The second involved the analysis of 53 yellow alleles, obtained from a variety of sources, with respect to complementation, molecular structure, and transcriptional competence. Our data suggest two ways in which transvection may be regulated at yellow: (1) a transcriptional mechanism, whereby the ability of an allele to support transvection is influenced by its transcriptional competency, and (2) a structural mechanism, whereby the pairing of structurally dissimilar homologues results in conformational changes that affect gene expression.

Details

Title: Subtitle: An Analysis of Transvection at the yellow Locus of Drosophila melanogaster
Creators: James R Morris - Harvard University
Ji-long Chen - University of Iowa
Stephen T Filandrinos - Harvard University
Rebecca C Dunn - University of Iowa
Ridgely Fisk - Harvard University
Pamela K Geyer - University of Iowa
Chao-ting Wu - Harvard University
Resource Type: Journal article
Publication Details: Genetics (Austin), Vol.151(2), pp.633-651
DOI: 10.1093/genetics/151.2.633
PMID: 9927457
PMCID: PMC1460495
NLM abbreviation: Genetics
ISSN: 1943-2631
eISSN: 1943-2631
Language: English
Date published: 02/01/1999
Academic Unit: Obstetrics and Gynecology; Biochemistry and Molecular Biology
Record Identifier: 9984293089502771

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