Journal article
An Equine Infectious Anemia Virus Variant Superinfects Cells through Novel Receptor Interactions
Journal of virology, Vol.82(19), pp.9425-9432
10/2008
DOI: 10.1128/JVI.01142-08
PMCID: PMC2546952
PMID: 18667522
Abstract
Wild-type strains of equine infectious anemia virus (EIAV) prevent superinfection of previously infected cells. A variant strain of virus that spontaneously arose during passage, EIAV
vMA-1c
, can circumvent this mechanism in some cells, such as equine dermis (ED) cells, but not in others, such as equine endothelial cells. EIAV
vMA-1c
superinfection of ED cells results in a buildup of unintegrated viral DNA and rapid killing of the cell monolayer. Here, we examined the mechanism of resistance that is used by EIAV to prevent superinfection and explored the means by which EIAV
vMA-1c
overcomes this restriction. We found that the cellular receptor used by EIAV, equine lentivirus receptor 1 (ELR1), remains on the surface of cells chronically infected with EIAV, suggesting that wild-type EIAV interferes with superinfection by masking ELR1. The addition of soluble wild-type SU protein to the medium during infection blocked infection by wild-type strains of virus, implicating SU as the viral protein responsible for interfering with virion entry into previously infected cells. Additionally, interference of wild-type EIAV binding to ELR1 by the addition of either anti-ELR1 antibodies or the ELR1 ectodomain prevented entry of the wild-type strains of EIAV into two permissive cell populations. Many of these same interference treatments prevented EIAV
vMA-1c
infection of endothelial cells but only modestly affected the ability of EIAV
vMA-1c
to enter and kill previously infected ED cells. These findings indicate that EIAV
vMA-1c
retains the ability to use ELR1 for entry and suggest that this virus can interact with an additional, unidentified receptor to superinfect ED cells.
Details
- Title: Subtitle
- An Equine Infectious Anemia Virus Variant Superinfects Cells through Novel Receptor Interactions
- Creators
- Melinda A Brindley - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242Baoshan Zhang - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242Ronald C Montelaro - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242Wendy Maury - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Journal of virology, Vol.82(19), pp.9425-9432
- Publisher
- American Society for Microbiology (ASM)
- DOI
- 10.1128/JVI.01142-08
- PMID
- 18667522
- PMCID
- PMC2546952
- ISSN
- 0022-538X
- eISSN
- 1098-5514
- Language
- English
- Date published
- 10/2008
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984083263402771
Metrics
15 Record Views