Journal article
An Exochelin of Mycobacterium tuberculosis Reversibly Arrests Growth of Human Vascular Smooth Muscle Cells in Vitro
The Journal of biological chemistry, Vol.275(23), pp.17821-17826
06/09/2000
DOI: 10.1074/jbc.M909918199
PMID: 10748174
Abstract
Proliferation of vascular smooth muscle cells (VSMC) is characteristic of restenosis following balloon angioplasty. We show here that a low concentration of a novel iron chelator, desferri-exochelin 772SM, reversibly arrests the growth of human VSMCin vitro, specifically in G0/G1 and S phases. The lipophilic desferri-exochelin is effective more rapidly and at a 10-fold lower concentration than the nonlipophilic iron chelator deferoxamine. Treatment of growth-synchronized VSMC with the desferri-exochelin results in down-regulation of cyclin E/ Cdk2 and cyclin A/Cdk2 activity but does not affect the cyclin D/Cdk4/retinoblastoma phosphorylation pathway. Both DNA replication and RNA transcription are inhibited in exochelin-treated cells, but protein synthesis is not. The ability of desferri-exochelin 772SM to reversibly block the growth of VSMC in vitro with no apparent cytotoxicity suggests that the exochelin may be useful as a therapeutic agent to limit restenosis in injured vessels.
Details
- Title: Subtitle
- An Exochelin of Mycobacterium tuberculosis Reversibly Arrests Growth of Human Vascular Smooth Muscle Cells in Vitro
- Creators
- Paula M.B. Pahl - University of Colorado HealthXiang-Dong Yan - University of Colorado HealthYvonne K. Hodges - University of Colorado HealthEli A. Rosenthal - University of Colorado HealthMarcus A. Horwitz - University of California, Los AngelesLawrence D. Horwitz - University of Colorado Health
- Resource Type
- Journal article
- Publication Details
- The Journal of biological chemistry, Vol.275(23), pp.17821-17826
- Publisher
- Elsevier Inc
- DOI
- 10.1074/jbc.M909918199
- PMID
- 10748174
- ISSN
- 0021-9258
- eISSN
- 1083-351X
- Language
- English
- Date published
- 06/09/2000
- Academic Unit
- Cardiovascular Medicine; Internal Medicine
- Record Identifier
- 9984656598802771
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