Journal article
An Open-Source Normothermic Perfusion System Designed for Research Scientists
Journal of Visualized Experiments, Vol.2025(221), e68722
07/18/2025
DOI: 10.3791/68722
PMID: 40758600
Abstract
The integration of ex vivo perfusion machines for human organs prior to transplantation has improved outcomes for recipients and increased organ availability while revealing novel avenues of investigation for translational medicine. However, these machines have limited availability for experimental modifications while presenting a significant upfront cost investment and prohibitive per-use cost to the research scientist. Furthermore, there is a significant need for improved pre-clinical models in biomedical research that allow for authentic interrogation of cellular processes in the multi-cellular organ setting. To provide access to similar technology for the greater research community, we constructed a cost-effective modular normothermic perfusion machine entirely from readily sourced parts run with user-modifiable Python code. Since the system is devoid of customized hardware, investigators can construct and augment the system as required for individualized research applications. Herein, we describe the machine set-up and perfusion process in detail, convey our experience with porcine and human organs, and provide early proof-of-concept experimental results. These studies demonstrate the potential to interrogate pathophysiology and complex cellular processes using intact human tissue.
Details
- Title: Subtitle
- An Open-Source Normothermic Perfusion System Designed for Research Scientists
- Creators
- Lindsay R. Friedman - National Institutes of HealthHannah E. Stepp - National Institutes of HealthJacob Lambdin - National Institutes of HealthAllen Luna - National Institutes of HealthAlexander Rossi - National Institutes of HealthStephanie C. Lux - National Institutes of HealthMichael M. Wach - National Institutes of HealthKenneth Luberice - National Institutes of HealthSamantha M. Ruff - National Institutes of HealthJames McDonald - National Institutes of HealthReed I. Ayabe - National Institutes of HealthShahyan U. Rehman - National Institutes of HealthCarrie Ryan - National Institutes of HealthStephanie Gregory - National Institutes of HealthAlyssa V. Eade - National Institutes of HealthCarolina Larrain - National Institutes of HealthJack H. Victory - National Institutes of HealthRandy Pursley - National Institute of Biomedical Imaging and BioengineeringTaylor Noel - National Institute of Biomedical Imaging and BioengineeringMax Freidlin - National Institute of Biomedical Imaging and BioengineeringShreya Gupta - National Institutes of HealthTracey Pu - National Institutes of HealthAshley Rainey - National Institutes of HealthEmily Verbus - National Institutes of HealthMartha Teke - National Institutes of HealthAreeba Saif - National Institutes of HealthTahsin M. Khan - National Institutes of HealthImani A. Alexander - National Institutes of HealthSurajit Sinha - National Institutes of HealthSarfraz R. Akmal - National Institutes of HealthRitish R. Maram - Society of Interventional RadiologyLeila Sarvestani - National Institutes of HealthStephen M. Hewitt - National Institutes of Health Clinical CenterJames Doroshow - National Institutes of HealthKirsten Remmert - National Institutes of HealthEmily Smith - National Institutes of HealthPriyanka Desai - National Institutes of HealthNing Miao - National Institutes of HealthKevin Driscoll - National Institutes of HealthKamille West - National Blood Transfusion ServiceTheo Heller - National Institute of Diabetes and Digestive and Kidney DiseasesElliot Levy - National Institutes of Health Clinical CenterJeremy Davis - National Institutes of HealthAndrew Blakely - National Institutes of HealthDavid E. Kleiner - National Institutes of Health Clinical CenterJohn Kakareka - National Institute of Biomedical Imaging and BioengineeringThomas Pohida - National Institute of Biomedical Imaging and BioengineeringJonathan M. Hernandez - National Cancer Institute
- Resource Type
- Journal article
- Publication Details
- Journal of Visualized Experiments, Vol.2025(221), e68722
- DOI
- 10.3791/68722
- PMID
- 40758600
- NLM abbreviation
- J Vis Exp
- ISSN
- 1940-087X
- eISSN
- 1940-087X
- Publisher
- MyJoVE Corporation
- Language
- English
- Date published
- 07/18/2025
- Academic Unit
- Surgery
- Record Identifier
- 9985224415802771
Metrics
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