An RNA Recognition Motif-Containing Protein Functions in Meiotic Silencing by Unpaired DNA

Dilini A Samarajeewa; Pennapa Manitchotpisit; Miranda Henderson; Hua Xiao; David G Rehard; Kevin A Edwards; Patrick K T Shiu; Thomas M Hammond

doi:10.1534/g3.117.041848

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An RNA Recognition Motif-Containing Protein Functions in Meiotic Silencing by Unpaired DNA

Journal article

Open access

An RNA Recognition Motif-Containing Protein Functions in Meiotic Silencing by Unpaired DNA

Dilini A Samarajeewa, Pennapa Manitchotpisit, Miranda Henderson, Hua Xiao, David G Rehard, Kevin A Edwards, Patrick K T Shiu and Thomas M Hammond

G3 (Bethesda, Md.), Vol.7(8), pp.2871-2882

08/07/2017

DOI: 10.1534/g3.117.041848

PMCID: PMC5555490

PMID: 28667016

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Abstract

Meiotic silencing by unpaired DNA (MSUD) is a biological process that searches pairs of homologous chromosomes (homologs) for segments of DNA that are unpaired. Genes found within unpaired segments are silenced for the duration of meiosis. In this report, we describe the identification and characterization of , a gene that encodes a protein with an RNA recognition motif (RRM). Orthologs of are found in a wide range of ascomycete fungi. In , is required for a fully efficient MSUD response to unpaired genes. Additionally, at least one parent must have a functional allele for a cross to produce ascospores. Although -null crosses are barren, strains grow at a wild-type (wt) rate and appear normal under vegetative growth conditions. With respect to expression, is transcribed at baseline levels in early vegetative cultures, at slightly higher levels in mating-competent cultures, and is at its highest level during mating. These findings suggest that SAD-7 is specific to mating-competent and sexual cultures. Although the role of SAD-7 in MSUD remains elusive, green fluorescent protein (GFP)-based tagging studies place SAD-7 within nuclei, perinuclear regions, and cytoplasmic foci of meiotic cells. This localization pattern is unique among known MSUD proteins and raises the possibility that SAD-7 coordinates nuclear, perinuclear, and cytoplasmic aspects of MSUD.

Fungal Proteins - chemistry

Gene Expression Regulation, Fungal

Amino Acid Sequence

Green Fluorescent Proteins - metabolism

Neurospora crassa - cytology

DNA, Fungal - genetics

Neurospora crassa - growth & development

Gene Silencing

Genes, Suppressor

Meiosis - genetics

Cell Nucleus - metabolism

Alleles

Neurospora crassa - genetics

RNA Recognition Motif

Spores, Fungal - genetics

Fungal Proteins - metabolism

Details

Title: Subtitle: An RNA Recognition Motif-Containing Protein Functions in Meiotic Silencing by Unpaired DNA
Creators: Dilini A Samarajeewa - School of Biological Sciences, Illinois State University, Normal, Illinois 61790
Pennapa Manitchotpisit - School of Biological Sciences, Illinois State University, Normal, Illinois 61790
Miranda Henderson - School of Biological Sciences, Illinois State University, Normal, Illinois 61790
Hua Xiao - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211
David G Rehard - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211
Kevin A Edwards - School of Biological Sciences, Illinois State University, Normal, Illinois 61790
Patrick K T Shiu - Division of Biological Sciences, University of Missouri, Columbia, Missouri 65211
Thomas M Hammond - School of Biological Sciences, Illinois State University, Normal, Illinois 61790 tmhammo@ilstu.edu
Resource Type: Journal article
Publication Details: G3 (Bethesda, Md.), Vol.7(8), pp.2871-2882
DOI: 10.1534/g3.117.041848
PMID: 28667016
PMCID: PMC5555490
NLM abbreviation: G3 (Bethesda)
ISSN: 2160-1836
eISSN: 2160-1836
Grant note: R15 HD076309 / NICHD NIH HHS P01 GM068087 / NIGMS NIH HHS
Language: English
Date published: 08/07/2017
Academic Unit: Biology
Record Identifier: 9984083266202771

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