Journal article
An adenoviral vector can transfer lacZ expression into schwann cells in culture and in sciatic nerve
Annals of neurology, Vol.38(3), pp.429-436
09/1995
DOI: 10.1002/ana.410380313
PMID: 7668829
Abstract
Although a number of genetic defects in the P0, peripheral myelin protein‐22, and connexin‐32 genes recently were shown to cause the demyelinating forms of Charcot‐Marie‐Tooth disease, there is yet no effective treatment for these patients. Recent studies showed that replication defective adenoviral vectors can efficiently introduce genes into muscle, brain, lung, and other tissues, suggesting that this vector system may be useful for the treatment of a number of genetic diseases. In this work, we demonstrated that a replication deficient adenovirus expressing the Escherichia coli β‐galactosidase gene (AdCMVLacZ) can introduce genes into Schwann cells, in culture as well as in sciatic nerve. Schwann cells cultured at a multiplicity of infection of 250:1 did not demonstrate cytopathic effects. Following injection of AdCMVLacZ into sciatic nerve of rats, lacZ‐expressing, myelinating Schwann cells could be detected for at least 45 days. These data suggest that in the future, these vectors may be useful both in perturbing Schwann cell gene expression and in designing therapies for the treatment of Charcot‐Marie‐Tooth disease.
Details
- Title: Subtitle
- An adenoviral vector can transfer lacZ expression into schwann cells in culture and in sciatic nerve
- Creators
- Michael E ShyMari TaniYi‐Jun ShiShelley A WhyattTaibi ChbihiSteven S SchererJohn Kamholz
- Resource Type
- Journal article
- Publication Details
- Annals of neurology, Vol.38(3), pp.429-436
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; Hoboken
- DOI
- 10.1002/ana.410380313
- PMID
- 7668829
- ISSN
- 0364-5134
- eISSN
- 1531-8249
- Number of pages
- 8
- Language
- English
- Date published
- 09/1995
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Psychiatry; Stead Family Department of Pediatrics; Iowa Neuroscience Institute
- Record Identifier
- 9984013205402771
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