Journal article
An apical-membrane chloride channel in human tracheal epithelium
Science (American Association for the Advancement of Science), Vol.232(4758), pp.1648-1650
1986
DOI: 10.1126/science.2424085
PMID: 2424085
Abstract
The mechanism of chloride transport by airway epithelia has been of substantial interest because airway and sweat gland-duct epithelia are chloride-impermeable in cystic fibrosis. The decreased chloride permeability prevents normal secretion by the airway epithelium, thereby interfering with mucociliary clearance and contributing to the morbidity and mortality of the disease. Because chloride secretion depends on and is regulated by chloride conductance in the apical cell membrane, the patch-clamp technique was used to directly examine single-channel currents in primary cultures of human tracheal epithelium. The cells contained an anion-selective channel that was not strongly voltage-gated or regulated by calcium in cell-free patches. The channel was also blocked by analogs of carboxylic acid that decrease apical chloride conductance in intact epithelia. When attached to the cell, the channel was activated by isoproterenol, although the channel was also observed to open spontaneously. However, in some cases, the channel was only observed after the patch was excised from the cell. These results suggest that this channel is responsible for the apical chloride conductance in airway epithelia.
Details
- Title: Subtitle
- An apical-membrane chloride channel in human tracheal epithelium
- Creators
- M. J WELSH - Univ. Iowa, coll. medicine, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Science (American Association for the Advancement of Science), Vol.232(4758), pp.1648-1650
- Publisher
- American Association for the Advancement of Science; Washington, DC
- DOI
- 10.1126/science.2424085
- PMID
- 2424085
- ISSN
- 0036-8075
- eISSN
- 1095-9203
- Language
- English
- Date published
- 1986
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Internal Medicine
- Record Identifier
- 9984020875402771
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