Journal article
An assay for the rate of removal of extracellular hydrogen peroxide by cells
Redox biology, Vol.1(1), pp.210-217
2013
DOI: 10.1016/j.redox.2013.01.011
PMCID: PMC3736862
PMID: 23936757
Abstract
Cells have a wide range of capacities to remove extracellular hydrogen peroxide. At higher concentrations of extracellular H
2
O
2
(micromolar) the rate of removal can be approximated by a rate equation that is first-order in the concentration of H
2
O
2
and cell density. Here we present a method to determine the observed rate constant for the removal of extracellular H
2
O
2
on a per cell basis. In the cells examined, when exposed to 20 μM H
2
O
2
, these rate constants (
k
cell
) range from 0.46×10
−12
s
−1
cell
−1
L for Mia-PaCa-2 cells (human pancreatic carcinoma) to 10.4×10
−12
s
−1
cell
−1
L for U937 cells (human histiocytic lymphoma). For the relatively small red blood cell
k
cell
=2.9×10
−12
s
−1
cell
−1
L. These rate constants,
k
cell
, can be used to compare the capacity of cells to remove higher levels of extracellular H
2
O
2
, as often presented in cell culture experiments. They also provide a means to estimate the rate of removal of extracellular H
2
O
2
, rate=−
k
cell
[H
2
O
2
] (cells L
−1
), and the half-life of a bolus of H
2
O
2
. This information is essential to optimize experimental design and interpret data from experiments that expose cells to extracellular H
2
O
2
.
Details
- Title: Subtitle
- An assay for the rate of removal of extracellular hydrogen peroxide by cells
- Creators
- Brett A Wagner - The University of Iowa, Free Radical and Radiation Biology Program & ESR Facility, Iowa City, IA 52242-1181, USAJordan R Witmer - The University of Iowa, Free Radical and Radiation Biology Program & ESR Facility, Iowa City, IA 52242-1181, USAThomas J van't Erve - The University of Iowa, Free Radical and Radiation Biology Program & ESR Facility, Iowa City, IA 52242-1181, USAGarry R Buettner - The University of Iowa, Free Radical and Radiation Biology Program & ESR Facility, Iowa City, IA 52242-1181, USA
- Resource Type
- Journal article
- Publication Details
- Redox biology, Vol.1(1), pp.210-217
- DOI
- 10.1016/j.redox.2013.01.011
- PMID
- 23936757
- PMCID
- PMC3736862
- NLM abbreviation
- Redox Biol
- ISSN
- 2213-2317
- eISSN
- 2213-2317
- Publisher
- Elsevier
- Language
- English
- Date published
- 2013
- Academic Unit
- Radiation Oncology
- Record Identifier
- 9984046932102771
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