An automated climbing apparatus to measure chemotherapy-induced neurotoxicity in Drosophila melanogaster

Jewel L Podratz; Nathan P Staff; Joshua B Boesche; Nicholas J Giorno; Matthew E Hainy; Shaun A Herring; Michael T Klennert; Christian Milaster; Steve E Nowakowski; Randall G Krug II; Ying Peng; Anthony J Windebank

doi:10.4161/fly.24789

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An automated climbing apparatus to measure chemotherapy-induced neurotoxicity in Drosophila melanogaster

Journal article

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An automated climbing apparatus to measure chemotherapy-induced neurotoxicity in Drosophila melanogaster

Jewel L Podratz, Nathan P Staff, Joshua B Boesche, Nicholas J Giorno, Matthew E Hainy, Shaun A Herring, Michael T Klennert, Christian Milaster, Steve E Nowakowski, Randall G Krug II, …

Fly (Austin, Tex.), Vol.7(3), pp.187-192

07/01/2013

DOI: 10.4161/fly.24789

PMID: 23695893

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Abstract

We have developed a novel model system in Drosophila melanogaster to study chemotherapy-induced neurotoxicity in adult flies. Neurological deficits were measured using a manual geotactic climbing assay. The manual assay is commonly used; however, it is laborious, time-consuming, subject to human error and limited to observing one sample at a time. We have designed and built a new automated fly-counting apparatus that uses a “video capture-particle counting technology” to automatically measure 10 samples at a time, with 20 flies per sample. Climbing behavior was assessed manually, as in our previous studies, and with the automated apparatus within the same experiment yielding statistically similar results. Both climbing endpoints as well as the climbing rate can be measured in the apparatus, giving the assay more versatility than the manual assay. Automation of our climbing assay reduces variability, increases productivity and enables high throughput drug screens for neurotoxicity.

Methods and Technical Advances

Details

Title: Subtitle: An automated climbing apparatus to measure chemotherapy-induced neurotoxicity in Drosophila melanogaster
Creators: Jewel L Podratz - Mayo Clinic
Nathan P Staff - Mayo Clinic
Joshua B Boesche - Mayo Clinic
Nicholas J Giorno - Mayo Clinic
Matthew E Hainy - Mayo Clinic
Shaun A Herring - Mayo Clinic
Michael T Klennert - Mayo Clinic
Christian Milaster - Mayo Clinic
Steve E Nowakowski - Mayo Clinic
Randall G Krug II
Ying Peng - Mayo Clinic
Anthony J Windebank - Mayo Clinic
Resource Type: Journal article
Publication Details: Fly (Austin, Tex.), Vol.7(3), pp.187-192
DOI: 10.4161/fly.24789
PMID: 23695893
NLM abbreviation: Fly (Austin)
ISSN: 1933-6934
eISSN: 1933-6942
Publisher: Landes Bioscience
Number of pages: 6
Language: English
Date published: 07/01/2013
Academic Unit: Neurology
Record Identifier: 9985240989602771

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