An efficient method for the extraction of DNA from formalin-fixed, paraffin-embedded tissue by sonication

M. J HELLER; L. J BURGART; C. J TENEYCK; M. E ANDERSON; T. C GREINER; R. A ROBINSON

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An efficient method for the extraction of DNA from formalin-fixed, paraffin-embedded tissue by sonication

Journal article

Peer reviewed

An efficient method for the extraction of DNA from formalin-fixed, paraffin-embedded tissue by sonication

M. J HELLER, L. J BURGART, C. J TENEYCK, M. E ANDERSON, T. C GREINER and R. A ROBINSON

BioTechniques, Vol.11(3), pp.372-377

1991

PMID: 1931037

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Abstract

A method was developed for fast and efficient isolation of DNA from formalin-fixed, paraffin-embedded tissue sections for subsequent use in PCRs and DNA hybridization assays. The method relies on the use of a sonicating water bath to disrupt tissue samples to which a small amount of micro-sized glass beads have been added. The sonicating glass beads provide fast and efficient physical shearing of fixed tissue sections, allowing for quick release and solubilization of the DNA. The extraction process from paraffin section to amplifiable target DNA takes 30 minutes. The method eliminates the need for repetitive solvent extractions and exhaustive proteinase K digestion. PCR amplification of human genomic and viral target sequences was successfully carried out on DNA isolated from a number of different types of normal and infected tissues.

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Diverse techniques

Molecular and cellular biology

Details

Title: Subtitle: An efficient method for the extraction of DNA from formalin-fixed, paraffin-embedded tissue by sonication
Creators: M. J HELLER - Univ. Iowa, coll. medicine, dep. pathology, Iowa City IA 52242, United States
L. J BURGART - Univ. Iowa, coll. medicine, dep. pathology, Iowa City IA 52242, United States
C. J TENEYCK - Univ. Iowa, coll. medicine, dep. pathology, Iowa City IA 52242, United States
M. E ANDERSON - Univ. Iowa, coll. medicine, dep. pathology, Iowa City IA 52242, United States
T. C GREINER - Univ. Iowa, coll. medicine, dep. pathology, Iowa City IA 52242, United States
R. A ROBINSON - Univ. Iowa, coll. medicine, dep. pathology, Iowa City IA 52242, United States
Resource Type: Journal article
Publication Details: BioTechniques, Vol.11(3), pp.372-377
Publisher: Eaton; Natick, MA
PMID: 1931037
ISSN: 0736-6205
eISSN: 1940-9818
Language: English
Date published: 1991
Academic Unit: Oral Pathology, Radiology and Medicine; Pathology
Record Identifier: 9984046810702771

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