Journal article
An enzyme's metal preference evolves through redox modulation driven by the cofactor's secondary coordination sphere
Molecular biology and evolution, Vol.43(3), msag040
03/2026
DOI: 10.1093/molbev/msag040
PMID: 41684149
Abstract
Changes in protein properties and functions are central to the evolution of life. Metalloproteins can evolve by changing their preference from one metal cofactor to another. Recently, we demonstrated that the widely distributed iron or manganese dependent superoxide dismutase (SodFM) family have undergone numerous metal-preference changes, including during evolutionary adaptation of pathogenic bacteria to altered metal availability within the host. Yet the underlying properties of metal-binding sites that control metalloenzyme metal-preference are unclear, and thus we lack an understanding of how enzymatic metal-preference can be re-shaped by evolution. Here, we used spectral features of bound iron or manganese, whose intensities reflect their oxidation state, to assess how their redox properties are tuned during SodFM evolution. We systematically analysed the metal oxidation state across diverse SodFMs from multiple phylogenetic groups with different catalytic metal-preferences, including those known to have undergone evolutionary metal-preference switching. We observed a striking relationship between resting oxidation state and catalytic metal-preferences. Mutagenesis of second-sphere residues previously identified as determining metal preference revealed that they modulate metal-dependent activity and cofactor oxidation state in tandem, demonstrating these properties are linked. Together, these data argue that the differing SodFM metal preferences observed across the tree of life evolved through tuning of their redox properties by the secondary coordination sphere. This study gives insight into the process by which a metalloenzyme originally optimised for one metal cofactor can evolve a new metal preference, under suitable selection pressure, through re-optimisation of its active site for catalytic reactivity of the new metal cofactor.
Details
- Title: Subtitle
- An enzyme's metal preference evolves through redox modulation driven by the cofactor's secondary coordination sphere
- Creators
- Eilidh S. Mackenzie - Newcastle UniversityKacper M. Sendra - Newcastle UniversityArnaud Basle - Newcastle UniversityRafał Mazgaj - Institute of Biochemistry and Biophysics, Polish Academy of SciencesThomas E Kehl-Fie - University of IowaKevin J Waldron - Institute of Biochemistry and Biophysics, Polish Academy of Sciences
- Resource Type
- Journal article
- Publication Details
- Molecular biology and evolution, Vol.43(3), msag040
- DOI
- 10.1093/molbev/msag040
- PMID
- 41684149
- NLM abbreviation
- Mol Biol Evol
- ISSN
- 0737-4038
- eISSN
- 1537-1719
- Publisher
- Oxford University Press
- Grant note
- Narodowe Centrum Nauki: 2021/42/A/NZ1/00214 Newcastle University's Faculty of Medical SciencesNational Institutes of Health: R01 AI155611 Biotechnology and Biological Sciences Research Council
E.S.M. was supported by a PhD studentship from the Biotechnology and Biological Sciences Research Council (BBSRC). K.M.S., K.J.W., and T.E.K.-F. were supported by a grant from the National Institutes of Health (R01 AI155611) to T.E.K.-F. K.J.W. was also funded by a Maestro grant from Narodowe Centrum Nauki (NCN), Poland (2021/42/A/NZ1/00214), which also supported R.M. A.B. was funded by the Newcastle University's Faculty of Medical Sciences.
- Language
- English
- Electronic publication date
- 02/13/2026
- Date published
- 03/2026
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9985139278202771
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