Journal article
An extracellular site on tetraspanin CD151 determines α3 and α6 integrin–dependent cellular morphology
The Journal of Cell Biology, Vol.158(7), pp.1299-1309
09/30/2002
DOI: 10.1083/jcb.200204056
PMCID: PMC2173251
PMID: 12356873
Abstract
The α3β1 integrin shows strong, stoichiometric, direct lateral association with the tetraspanin CD151. As shown here, an extracellular CD151 site (QRD
194–196
) is required for strong (i.e., Triton X-100–resistant) α3β1 association and for maintenance of a key CD151 epitope (defined by monoclonal antibody TS151r) that is blocked upon α3 integrin association. Strong CD151 association with integrin α6β1 also required the QRD
194–196
site and masked the TS151r epitope. For both α3 and α6 integrins, strong QRD/TS151r-dependent CD151 association occurred early in biosynthesis and involved α subunit precursor forms. In contrast, weaker associations of CD151 with itself, integrins, or other tetraspanins (Triton X-100–sensitive but Brij 96–resistant) were independent of the QRD/TS151r site, occurred late in biosynthesis, and involved mature integrin subunits. Presence of the CD151–QRD
194–196
→INF mutant disrupted α3 and α6 integrin–dependent formation of a network of cellular cables by Cos7 or NIH3T3 cells on basement membrane Matrigel and markedly altered cell spreading. These results provide definitive evidence that strong lateral CD151–integrin association is functionally important, identify CD151 as a key player during α3 and α6 integrin–dependent matrix remodeling and cell spreading, and support a model of CD151 as a transmembrane linker between extracellular integrin domains and intracellular cytoskeleton/signaling molecules.
Details
- Title: Subtitle
- An extracellular site on tetraspanin CD151 determines α3 and α6 integrin–dependent cellular morphology
- Creators
- Alexander R Kazarov - Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115Xiuwei Yang - Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115Christopher S Stipp - Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115Bantoo Sehgal - Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115Martin E Hemler - Dana-Farber Cancer Institute and Department of Pathology, Harvard Medical School, Boston, MA 02115
- Resource Type
- Journal article
- Publication Details
- The Journal of Cell Biology, Vol.158(7), pp.1299-1309
- Publisher
- The Rockefeller University Press
- DOI
- 10.1083/jcb.200204056
- PMID
- 12356873
- PMCID
- PMC2173251
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 09/30/2002
- Academic Unit
- Molecular Physiology and Biophysics; Biology
- Record Identifier
- 9983991981702771
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