Journal article
An improved large-scale synthesis of PEG-peptides for gene delivery
The journal of peptide research, Vol.64(6), pp.237-243
12/2004
DOI: 10.1111/j.1399-3011.2004.00195.x
PMID: 15613087
Abstract
Polyethylene glycol (PEG)-peptides are under development as components of nonviral gene delivery systems. Several earlier reports have demonstrated that covalent attachment of PEG to the surface of peptide condensed DNA particles blocks non-specific biodistribution during gene targeting. In this study, we report an improved large-scale synthesis and purification of a DNA condensing PEG-peptide used for gene delivery. The new method takes advantage of low-pressure cation-exchange chromatography to isolate dimeric Cys-Trp-Lys(18). The dimeric peptide was reduced and directly conjugated with PEG-maleimide resulting in PEG-Cys-Trp-Lys(18). The PEG-peptide was purified by low-pressure chromatography affording 50 mumol (400 mg) quantities of PEG-peptide in >95% purity. The approach offers the advantage of avoiding preparative high-performance liquid chromatography (HPLC) purifications of polylysine peptides to increase yield and capacity.
Details
- Title: Subtitle
- An improved large-scale synthesis of PEG-peptides for gene delivery
- Creators
- C-P Chen - Division of Medicinal and Natural Products Chemistry, College of Pharmacy, The University of Iowa, 115 South Grand Avenue, Iowa City, IA 52242, USAY ParkK G Rice
- Resource Type
- Journal article
- Publication Details
- The journal of peptide research, Vol.64(6), pp.237-243
- DOI
- 10.1111/j.1399-3011.2004.00195.x
- PMID
- 15613087
- NLM abbreviation
- J Pept Res
- ISSN
- 1397-002X
- eISSN
- 1399-3011
- Publisher
- Denmark
- Grant note
- DK063196 / NIDDK NIH HHS
- Language
- English
- Date published
- 12/2004
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984065314502771
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