Journal article
An in vitro model for studies of attenuation of antibiotic-inhibited growth of Aggregatibacter actinomycetemcomitans Y4 by polyamines
Molecular oral microbiology, Vol.36(6), pp.308-315
12/01/2021
DOI: 10.1111/omi.12353
PMCID: PMC9293220
PMID: 34486245
Abstract
Polyamines are ubiquitous polycationic molecules that are present in all prokaryotic and eukaryotic cells, and they serve as important modulators of cell growth, stress, and cell proliferation. Polyamines are present at high concentrations in the periodontal pocket and could potentially affect the stress response of periodontal bacteria to antibiotics. The effects of polyamines on inhibition of growth by amoxicillin (AMX), azithromycin (AZM), and doxycycline (DOX) were investigated with the Y4 strain of Aggregatibacter actinomycetemcomitans (Aa). Bacteria were grown in brain heart infusion broth under the following conditions: (1) Aa only, (2) Aa + polyamine mix (1 mM putrescine, 0.4 mM spermidine, and 0.4 mM spermine), (3) Aa + antibiotic, and (4) Aa + antibiotic + polyamines. Growth curve analysis, minimal inhibitory concentration determination, and transcriptomic studies were conducted. The presence of exogenous polyamines produced a small, but significant increase in Aa growth, and polyamines attenuated the inhibitory effects of AMX, AZM, and DOX on growth. Transcriptomic analysis revealed that polyamines upregulate expression of ribosomal biogenesis proteins and small subunits, attenuate the bacterial stress response to antibiotics, and modulate bacterial nutritional pathways in a manner that could potentially increase the virulence of Aa. In summary, the polyamine-rich environment found in periodontal pockets appears to protect Aa and reduce its susceptibility to several antimicrobial agents in this in vitro model.
Details
- Title: Subtitle
- An in vitro model for studies of attenuation of antibiotic-inhibited growth of Aggregatibacter actinomycetemcomitans Y4 by polyamines
- Creators
- Allan Wattimena - The Ohio State UniversitySukirth M. Ganesan - The Ohio State UniversityPurnima S. Kumar - The Ohio State UniversityShareef M. Dabdoub - The Ohio State UniversityJohn D. Walters - The Ohio State University
- Resource Type
- Journal article
- Publication Details
- Molecular oral microbiology, Vol.36(6), pp.308-315
- DOI
- 10.1111/omi.12353
- PMID
- 34486245
- PMCID
- PMC9293220
- NLM abbreviation
- Mol Oral Microbiol
- ISSN
- 2041-1006
- eISSN
- 2041-1014
- Publisher
- Wiley
- Number of pages
- 8
- Grant note
- Office of Research in The Ohio State University College of Dentistry
- Language
- English
- Date published
- 12/01/2021
- Academic Unit
- Dental Research; Periodontics
- Record Identifier
- 9984367733302771
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