Journal article
An in vitro model of differentiated human airway epithelia. Methods for establishing primary cultures
Methods in molecular biology (Clifton, N.J.), Vol.188, pp.115-137
2002
DOI: 10.1385/1-59259-185-X:115
PMID: 11987537
Abstract
The human airway epithelium forms a barrier between the external and internal environments, separating air from the interstitial space. However, it also serves many other functions. By active transepithelial transport of electrolytes, it controls the composition and quantity of the airway surface liquid covering the epithelium. It secretes numerous agents into the airway surface liquid, including IgA and antimicrobial factors; these form part of the defensive shield that protects the airways and lungs from infection. The activity of its cilia are key to mucociliary clearance. The epithelium participates in the inflammatory response when challenged with environmental factors or infectious agents. It responds to and produces a number of cytokines and other pro- and antiinflammatory agents. To study and understand the complex and varied functions of human airway epithelia, investigators have developed cell culture models of the epithelium. Compared to in vivo studies, such models have the important advantage of flexibility, control of experimental conditions, and greater opportunities for intervention. They also allow the study of epithelial function in the absence of other cells and tissues such as macrophages, submucosal glands, fibroblasts, and cells of the immune system. Conversely, for some studies, the presence of nonepithelial cells and tissues would be advantageous.
Details
- Title: Subtitle
- An in vitro model of differentiated human airway epithelia. Methods for establishing primary cultures
- Creators
- Philip H Karp - Howard Hughes Medical Institute, Department of Internal Medicine, University of Iowa College of Medicine, Iowa City, IA, USAThomas O MoningerS Pary WeberTamara S NesselhaufJanice L LaunspachJoseph ZabnerMichael J Welsh
- Resource Type
- Journal article
- Publication Details
- Methods in molecular biology (Clifton, N.J.), Vol.188, pp.115-137
- DOI
- 10.1385/1-59259-185-X:115
- PMID
- 11987537
- ISSN
- 1064-3745
- eISSN
- 1940-6029
- Language
- English
- Date published
- 2002
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Fraternal Order of Eagles Diabetes Research Center; Neurosurgery; Internal Medicine
- Record Identifier
- 9984094522102771
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