Journal article
An in vitro study of a novel quaternary ammonium silane endodontic irrigant
Dental materials, Vol.35(9), pp.1264-1278
09/2019
DOI: 10.1016/j.dental.2019.05.020
PMID: 31201019
Abstract
To analyze effect of NaOCl+2% quaternary ammonium silane (QAS)-containing novel irrigant against bacteria impregnated inside the root canal system, and to evaluate its antimicrobial and mechanical potential of dentine substrate.
Root canal was prepared using stainless steel K-files™ and ProTaper™ and subjected to manual and ultrasonic irrigation using 6% NaOCl+2% CHX, 6% NaOCl+2% QAS and saline as control. For confocal-microscopy, Raman spectroscopy and SEM analysis before and after treatment, Enterococcus faecalis cultured for 7 days. Raman spectroscopy analysis was done across cut section of gutta percha/sealer-dentine to detect resin infiltration. Indentation of mechanical properties was evaluated using a Berkovich indenter. The contact angle of irrigants and surface free energy were evaluated. Mineralization nodules were detected through Alazarin red after 14 days.
Control biofilms showed dense green colonies. Majority of E. faecalis bacteria were present in biofilm fluoresced red in NaOCl+2% QAS group. There was reduction of 484cm−1 Raman band and its intensity reached lowest with NaOCl+2% QAS. There was an increase in 1350–1420cm−1 intensity in the NaOCl+2% CHX groups. Gradual decrease in 1639cm−1 and 1609cm−1 Raman signal ratios were seen in the resin-depth region of 17μm>, 14.1μm> and 13.2μm for NaOCl+2% QAS, NaOCl+2% CHX and control groups respectively. All obturated groups showed an intact sealer/dentine interface with a few notable differences. 0.771 and 83.5% creep indentation distance for NaOCl+2% QAS ultrasonic groups were observed. Highest proportion of polar component was significantly found in the NaOCl+2% QAS groups which was significantly higher as compared to other groups. Mineralized nodules were increased in NaOCl+2% QAS.
Favorable antimicrobial and endodontic profile of the NaOCl+2% QAS solution might suggest clinical use for it for more predictable reduction of intracanal bacteria.
Details
- Title: Subtitle
- An in vitro study of a novel quaternary ammonium silane endodontic irrigant
- Creators
- U. Daood - International Medical UniversityA. Parolia - International Medical UniversityA. Elkezza - International Medical UniversityC.K. Yiu - Prince Philip Dental HospitalP. Abbott - University of Western AustraliaJ.P. Matinlinna - University of Hong KongA.S. Fawzy - University of Western Australia
- Resource Type
- Journal article
- Publication Details
- Dental materials, Vol.35(9), pp.1264-1278
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.dental.2019.05.020
- PMID
- 31201019
- ISSN
- 0109-5641
- eISSN
- 1879-0097
- Grant note
- DOI: 10.13039/501100009381, name: International Medical University
- Language
- English
- Date published
- 09/2019
- Academic Unit
- Endodontics
- Record Identifier
- 9984446534902771
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