Journal article
An inducible model of abacterial prostatitis induces antigen specific inflammatory and proliferative changes in the murine prostate
The Prostate, Vol.71(11), pp.1139-1150
08/01/2011
DOI: 10.1002/pros.21327
PMCID: PMC3136647
PMID: 21656824
Abstract
Background: Prostatitis is a poorly understood disease and increasing evidence suggests inflammation is involved in other prostatic diseases including prostate cancer.
Methods: The ability of pre-activated CD8 T cells to induce prostatitis was examined by adoptive transfer of prostate antigen specific CD8 T cells into POET-3 mice or POET-3/Luc/Pten(-/+) mice. Characterization of the inflammatory response was determined by examining leukocyte infiltration by histological analysis, flow cytometry and by evaluating cytokine and chemokine levels in prostate tissue. The impact of inflammation on the prostate was evaluated by monitoring epithelial cell proliferation over time.
Results: Initiation of inflammation by ovalbumin specific CD8⁺ T cells (OT-I cells) resulted in development of acute prostatitis in the anterior, dorsolateral and ventral prostate of POET-3 and POET-3/Luc/Pten(-/+) mice. Acute prostatitis was characterized by recruitment of adoptively transferred OT-I cells and importantly, autologous CD4⁺ and CD8⁺ T cells, myeloid-derived suppressor cells (MDSC) and regulatory T cells (Treg). In concert with leukocyte infiltration elevated levels of pro-inflammatory cytokines and chemokines were observed. Inflammation also resulted in marked epithelial cell proliferation that was sustained up to 80 days post adoptive transfer of OT-I cells.
Conclusions: The POET-3 model represents a novel mouse model to study both acute and chronic prostate inflammation in an antigen-specific system. Further, the POET-3 mouse model can be crossed with other genetic models of disease such as the C57/Luc/Pten(-/-) model of prostate cancer, allowing the impact of prostatitis on other prostatic diseases to be evaluated.
Details
- Title: Subtitle
- An inducible model of abacterial prostatitis induces antigen specific inflammatory and proliferative changes in the murine prostate
- Creators
- Jessica M Haverkamp - Department of Comparative Pathobiology, Purdue University, West Lafayette, IndianaBridget Charbonneau - University of Iowa Microbiology Program, Iowa City, IowaScott A Crist - Department of Comparative Pathobiology, Purdue University, West Lafayette, IndianaDavid K Meyerholz - Department of Pathology, The University of Iowa, Iowa City, IowaMichael B Cohen - Departments of Pathology, Urology and Epidemiology, University of Iowa Carver College of Medicine, Iowa City, IowaPaul W Snyder - Department of Comparative Pathobiology, Purdue University, West Lafayette, IndianaRobert U Svensson - Department of Molecular Physiology and Biophysics, The University of Iowa, Iowa City, IowaMichael D Henry - Departments of Molecular Physiology and Biophysics and Pathology, University of Iowa Carver College of Medicine, Iowa City, IowaHsing-Hui Wang - Department of Comparative Pathobiology, Purdue University, West Lafayette, IndianaTimothy L Ratliff - Department of Comparative Pathobiology, Purdue University, West Lafayette, Indiana
- Resource Type
- Journal article
- Publication Details
- The Prostate, Vol.71(11), pp.1139-1150
- DOI
- 10.1002/pros.21327
- PMID
- 21656824
- PMCID
- PMC3136647
- NLM abbreviation
- Prostate
- ISSN
- 0270-4137
- eISSN
- 1097-0045
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- Number of pages
- 12
- Grant note
- name: NIH, award: DL084454
- Language
- English
- Date published
- 08/01/2011
- Academic Unit
- Molecular Physiology and Biophysics; Pathology; Radiation Oncology; Urology; Internal Medicine
- Record Identifier
- 9984083227302771
Metrics
37 Record Views