Journal article
An inexpensive microcomputer digital imaging system for densitometry: quantitative autoradiography of insulin receptors with 125I and LKB ultrofilm
Journal of neuroscience methods, Vol.16(2), pp.119-129
1986
DOI: 10.1016/0165-0270(86)90044-0
PMID: 3755201
Abstract
This article describes a video digitizing system designed for measuring film optical density. The system, which is based on a 6-bit (64 gray level) digitizer, solid state video camera, and Apple II microcomputer, digitizes a rectangular area selected by the operator and converts the gray level values into preselected standard units. In order to develop autoradiographic standard curves for quantitative autoradiography with
125I-insulin, liver slices labeled with
125I-insulin and plastic sections containing known amounts of tritium were apposed to the same sheet of LKB Ultrofilm for exposures of 1–7 days. The results indicate that
3H plastic standards can be used to calibrate QAR of
125I-labeled ligands with LKB Ultrofilm. The Apple system was also used to measure binding of
125I-insulin to the external plexiform layer (EPL) in slices of the rat olfactory bulb. Results suggest that the EPL has two binding sites for insulin, a high affinity site with
K
d
= 1.0 × 10
−8 M and a low affinity site having a
K
d
= 1.4 × 10
−5 M.
Details
- Title: Subtitle
- An inexpensive microcomputer digital imaging system for densitometry: quantitative autoradiography of insulin receptors with 125I and LKB ultrofilm
- Creators
- Denis G. Baskin - University of WashingtonDavid Davidson - University of WashingtonEric S. Corp - University of WashingtonThomas Lewellen - University of WashingtonMichael Graham - University of Washington
- Resource Type
- Journal article
- Publication Details
- Journal of neuroscience methods, Vol.16(2), pp.119-129
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0165-0270(86)90044-0
- PMID
- 3755201
- ISSN
- 0165-0270
- eISSN
- 1872-678X
- Language
- English
- Date published
- 1986
- Academic Unit
- Radiology; Radiation Oncology
- Record Identifier
- 9984312974202771
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