Journal article
An inflammatory equine model demonstrates dynamic changes of immune response and cartilage matrix molecule degradation in vitro
Connective tissue research, Vol.56(4), pp.315-325
2015
DOI: 10.3109/03008207.2015.1027340
PMID: 25803623
Abstract
The molecular aspects of inflammation were investigated in equine articular cartilage explants using quantitative proteomics. Articular cartilage explants were stimulated with interleukin (IL)-1 beta in vitro for 25 days, and proteins released into cell culture media were chemically labeled with isobaric mass tags and analyzed by liquid chromatography-tandem mass spectrometry. A total of 127 proteins were identified and quantified in media from explants. IL-1 beta-stimulation resulted in an abundance of proteins related to inflammation, including matrix metalloproteinases, acute phase proteins, complement components and IL-6. Extracellular matrix (ECM) molecules were released at different time points, and fragmentation of aggrecan and cartilage oligomeric matrix protein was observed at days 3 and 6, similar to early-stage OA in vivo. Degradation products of the collagenous network were observed at days 18 and 22, similar to late-stage OA. This model displays a longitudinal quantification of released molecules from the ECM of articular cartilage. Identification of dynamic changes of extracellular matrix molecules in the secretome of equine explants stimulated with IL-1 beta over time may be useful for identifying components released at different time points during the spontaneous OA process.
Details
- Title: Subtitle
- An inflammatory equine model demonstrates dynamic changes of immune response and cartilage matrix molecule degradation in vitro
- Creators
- Emilia Svala - Swedish University of Agricultural SciencesMaria Lofgren - Swedish University of Agricultural SciencesCarina Sihlbom - University of GothenburgUlla Ruetschi - Sahlgrenska University HospitalAnders Lindahl - Sahlgrenska University HospitalStina Ekman - Swedish University of Agricultural SciencesEva Skioldebrand - Swedish University of Agricultural Sciences
- Resource Type
- Journal article
- Publication Details
- Connective tissue research, Vol.56(4), pp.315-325
- Publisher
- Taylor & Francis
- DOI
- 10.3109/03008207.2015.1027340
- PMID
- 25803623
- ISSN
- 0300-8207
- eISSN
- 1607-8438
- Number of pages
- 11
- Grant note
- FORMAS 221-2013-317 / Swedish Research Council Formas for Environment, Agricultural Sciences and Spatial Planning H0947014 / Swedish-Norwegian Foundation for Equine Research
- Language
- English
- Date published
- 2015
- Academic Unit
- Nursing
- Record Identifier
- 9984370649102771
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