Journal article
An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding
Proceedings of the National Academy of Sciences - PNAS, Vol.92(17), pp.8011-8015
08/15/1995
DOI: 10.1073/pnas.92.17.8011
PMCID: PMC41276
PMID: 7644530
Abstract
We have isolated a major integral membrane protein from Golgi-derived coatomer-coated vesicles. This 24-kDa protein, p24, defines a family of integral membrane proteins with homologs present in yeast and humans. In addition to sequence similarity, all p24 family members contain a motif with the characteristic heptad repeats found in coiled coils. When the yeast p24 isoform, yp24A, is knocked out in a strain defective for vesicle fusion, a dramatic reduction in the accumulation of transport vesicles is observed. Together, these results indicate a role for this protein family in the budding of coatamer-coated and other species of coated vesicles.
Details
- Title: Subtitle
- An integral membrane component of coatomer-coated transport vesicles defines a family of proteins involved in budding
- Creators
- Mark A Stamnes - Memorial Sloan Kettering Cancer CenterMark W Craighead - Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USAMee H Hoe - Memorial Sloan Kettering Cancer CenterNina Lampen - Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USAScott Geromanos - Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USAPaul Tempst - Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USAJames E Rothman - Cellular Biochemistry and Biophysics Program, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.92(17), pp.8011-8015
- DOI
- 10.1073/pnas.92.17.8011
- PMID
- 7644530
- PMCID
- PMC41276
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 08/15/1995
- Academic Unit
- Molecular Physiology and Biophysics; Internal Medicine
- Record Identifier
- 9984297494102771
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