An interbacterial DNA deaminase toxin directly mutagenizes surviving target populations

Marcos H. de Moraes; FoSheng Hsu; Dean Huang; Dustin E Bosch; Jun Zeng; Matthew C. Radey; Noah Simon; Hannah E. Ledvina; Jacob P Frick; Paul A. Wiggins; S. Brook Peterson; Joseph D. Mougous

doi:10.7554/eLife.62967

Back

An interbacterial DNA deaminase toxin directly mutagenizes surviving target populations

Journal article

Open access

Peer reviewed

An interbacterial DNA deaminase toxin directly mutagenizes surviving target populations

Marcos H. de Moraes, FoSheng Hsu, Dean Huang, Dustin E Bosch, Jun Zeng, Matthew C. Radey, Noah Simon, Hannah E. Ledvina, Jacob P Frick, Paul A. Wiggins, …

eLife, Vol.10, e62967

01/01/2021

DOI: 10.7554/eLife.62967

PMCID: PMC7901873

PMID: 33448264

Files and links (1)

url

https://doi.org/10.7554/eLife.62967View

Published (Version of record) Open Access

Abstract

When bacterial cells come in contact, antagonism mediated by the delivery of toxins frequently ensues. The potential for such encounters to have long-term beneficial consequences in recipient cells has not been investigated. Here, we examined the effects of intoxication by DddA, a cytosine deaminase delivered via the type VI secretion system (T6SS) of Burkholderia cenocepacia. Despite its killing potential, we observed that several bacterial species resist DddA and instead accumulate mutations. These mutations can lead to the acquisition of antibiotic resistance, indicating that even in the absence of killing, interbacterial antagonism can have profound consequences on target populations. Investigation of additional toxins from the deaminase superfamily revealed that mutagenic activity is a common feature of these proteins, including a representative we show targets single-stranded DNA and displays a markedly divergent structure. Our findings suggest that a surprising consequence of antagonistic interactions between bacteria could be the promotion of adaptation via the action of directly mutagenic toxins.

Bacteria

Developmental Biology

DNA

Microbiology

Urogenital System

Adaptation

Antagonism

basic medicine

Burkholderia

Burkholderia cenocepacia

Cytosine deaminase

food and beverages

fungi

humanities

medical and health sciences

reproductive and urinary physiology

Toxin

Type VI secretion system

Details

Title: Subtitle: An interbacterial DNA deaminase toxin directly mutagenizes surviving target populations
Creators: Marcos H. de Moraes
FoSheng Hsu
Dean Huang
Dustin E Bosch
Jun Zeng
Matthew C. Radey
Noah Simon
Hannah E. Ledvina
Jacob P Frick
Paul A. Wiggins
S. Brook Peterson
Joseph D. Mougous
Resource Type: Journal article
Publication Details: eLife, Vol.10, e62967
DOI: 10.7554/eLife.62967
PMID: 33448264
PMCID: PMC7901873
NLM abbreviation: Elife
eISSN: 2050-084X
Publisher: eLife Sciences Publications, Ltd
Grant note: DOI: 10.13039/100000002, name: National Institutes of Health, award: GM128191; DOI: 10.13039/100000002, name: National Institutes of Health, award: AI080609; DOI: 10.13039/100000011, name: Howard Hughes Medical Institute; DOI: 10.13039/100000897, name: Cystic Fibrosis Foundation, award: DEMORA18F0; DOI: 10.13039/100000861, name: Burroughs Wellcome Fund; DOI: 10.13039/100000002, name: National Institutes of Health, award: DK089507; DOI: 10.13039/100000897, name: Cystic Fibrosis Foundation, award: SINGH19RO
Language: English
Date published: 01/01/2021
Academic Unit: Pathology
Record Identifier: 9984530266202771

Metrics

31 Record Views

39 Times Cited - Web of Science