Journal article
An open-source toolbox for automated phenotyping of mice in behavioral tasks
Frontiers in behavioral neuroscience, Vol.8, pp.349-349
10/08/2014
DOI: 10.3389/fnbeh.2014.00349
PMCID: PMC4189437
PMID: 25339878
Abstract
Classifying behavior patterns in mouse models of neurological, psychiatric and neurodevelopmental disorders is critical for understanding disease causality and treatment. However, complete characterization of behavior is time-intensive, prone to subjective scoring, and often requires specialized equipment. Although several reports describe automated home-cage monitoring and individual task scoring methods, we report the first open source, comprehensive toolbox for automating the scoring of several common behavior tasks used by the neuroscience community. We show this new toolbox is robust and achieves equal or better consistency when compared to manual scoring methods. We use this toolbox to study the alterations in behavior that occur following blast-induced traumatic brain injury (bTBI), and study if these behavior patterns are altered following genetic deletion of the transcription factor Ets-like kinase 1 (Elk-1). Due to the role of Elk-1 in neuronal survival and proposed role in synaptic plasticity, we hypothesized that Elk-1 deletion would improve some neurobehavioral deficits, while impairing others, following blast exposure. In Elk-1 knockout (KO) animals, deficits in open field, spatial object recognition (SOR) and elevated zero maze performance after blast exposure disappeared, while new significant deficits appeared in spatial and associative memory. These are the first data suggesting a molecular mediator of anxiety deficits following bTBI, and represent the utility of the broad screening tool we developed. More broadly, we envision this open-source toolbox will provide a more consistent and rapid analysis of behavior across many neurological diseases, promoting the rapid discovery of novel pathways mediating disease progression and treatment.
Details
- Title: Subtitle
- An open-source toolbox for automated phenotyping of mice in behavioral tasks
- Creators
- Tapan P Patel - Department of Bioengineering, University of PennsylvaniaDavid M Gullotti - Department of Bioengineering, University of PennsylvaniaPepe Hernandez - Department of Biology, University of PennsylvaniaW. Timothy O'Brien - Department of Neuroscience, University of PennsylvaniaBruce P Capehart - Department of Psychiatry and Behavioral Sciences, Duke UniversityBarclay Morrison - Department of Biomedical Engineering, Columbia UniversityCameron Bass - Department of Biomedical Engineering, Duke UniversityJames E Eberwine - Department of Pharmacology, University of PennsylvaniaTed Abel - Department of Biology, University of PennsylvaniaDavid F Meaney - Department of Bioengineering, University of Pennsylvania
- Resource Type
- Journal article
- Publication Details
- Frontiers in behavioral neuroscience, Vol.8, pp.349-349
- DOI
- 10.3389/fnbeh.2014.00349
- PMID
- 25339878
- PMCID
- PMC4189437
- NLM abbreviation
- Front Behav Neurosci
- ISSN
- 1662-5153
- eISSN
- 1662-5153
- Publisher
- Frontiers Media S.A
- Language
- English
- Date published
- 10/08/2014
- Academic Unit
- Molecular Physiology and Biophysics; Psychiatry; Psychological and Brain Sciences; Iowa Neuroscience Institute; Neuroscience and Pharmacology; Biochemistry and Molecular Biology
- Record Identifier
- 9984065829902771
Metrics
28 Record Views