Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect–parasite relationship

Rod J Dillon; Al C Ivens; Carol Churcher; Nancy Holroyd; Michael A Quail; Matthew E Rogers; M. Bento Soares; Maria F Bonaldo; Thomas L Casavant; Mike J Lehane; Paul A Bates

doi:10.1016/j.ygeno.2006.06.011

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Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect–parasite relationship

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Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect–parasite relationship

Rod J Dillon, Al C Ivens, Carol Churcher, Nancy Holroyd, Michael A Quail, Matthew E Rogers, M. Bento Soares, Maria F Bonaldo, Thomas L Casavant, Mike J Lehane, …

Genomics (San Diego, Calif.), Vol.88(6), pp.831-840

12/2006

DOI: 10.1016/j.ygeno.2006.06.011

PMCID: PMC2675706

PMID: 16887324

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Abstract

An expressed sequence tag library has been generated from a sand fly vector of visceral leishmaniasis, Lutzomyia longipalpis. A normalized cDNA library was constructed from whole adults and 16,608 clones were sequenced from both ends and assembled into 10,203 contigs and singlets. Of these 58% showed significant similarity to known genes from other organisms, <4% were identical to described sand fly genes, and 42% had no match to any database sequence. Our analyses revealed putative proteins involved in the barrier function of the gut (peritrophins, microvillar proteins, glutamine synthase), digestive physiology (secreted and membrane-anchored hydrolytic enzymes), and the immune response (gram-negative binding proteins, thioester proteins, scavenger receptors, galectins, signaling pathway factors, caspases, serpins, and peroxidases). Sequence analysis of this transcriptome dataset has provided new insights into genes that might be associated with the response of the vector to the development of Leishmania.

Midgut

Leishmania

Genomics

Lutzomyia

Parasite

Expressed sequence tag

Immunity

Details

Title: Subtitle: Analysis of ESTs from Lutzomyia longipalpis sand flies and their contribution toward understanding the insect–parasite relationship
Creators: Rod J Dillon - Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
Al C Ivens - The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
Carol Churcher - The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
Nancy Holroyd - The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
Michael A Quail - The Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, UK
Matthew E Rogers - Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
M. Bento Soares - Children's Memorial Research Center and Northwestern University, Chicago, IL 60611, USA
Maria F Bonaldo - Children's Memorial Research Center and Northwestern University, Chicago, IL 60611, USA
Thomas L Casavant - Department of Electrical and Computer Engineering, University of Iowa, Iowa City, IA 52242, USA
Mike J Lehane - Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
Paul A Bates - Liverpool School of Tropical Medicine, Pembroke Place, Liverpool L3 5QA, UK
Resource Type: Journal article
Publication Details: Genomics (San Diego, Calif.), Vol.88(6), pp.831-840
DOI: 10.1016/j.ygeno.2006.06.011
PMID: 16887324
PMCID: PMC2675706
NLM abbreviation: Genomics
ISSN: 0888-7543
eISSN: 1089-8646
Publisher: Elsevier Inc
Language: English
Date published: 12/2006
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering
Record Identifier: 9984064257602771

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