Journal article
Analysis of NLRP3 in the Development of Allergic Airway Disease in Mice
The Journal of immunology (1950), Vol.188(6), pp.2884-2893
03/15/2012
DOI: 10.4049/jimmunol.1102488
PMCID: PMC3294123
PMID: 22323538
Abstract
The contribution of NLRP3, a member of the nucleotide-binding domain leucine-rich repeat-containing (NLR) family, to the development of allergic airway disease is currently controversial. In this study, we used multiple allergic asthma models to examine the physiologic role of NLRP3. We found no significant differences in airway eosinophilia, histopathologic condition, mucus production, and airway hyperresponsiveness between wild-type and Nlrp3(-/-) mice in either acute (alum-dependent) or chronic (alum-independent) OVA models. In addition to the OVA model, we did not detect a role for NLRP3 in the development of allergic airway disease induced by either acute or chronic house dust mite Ag exposure. Although we did not observe significant phenotypic differences in any of the models tested, we did note a significant reduction of IL-13 and IL-33 in Nlrp3(-/-) mice compared with wild-type controls in the chronic OVA model without added alum. In all of the allergic airway disease models, the NLRP3 inflammasome-associated cytokines IL-1 beta and IL-18 in the lung were below the level of detection. In sum, this report surveyed four different allergic asthma models and found a modest and selected role for NLRP3 in the alum-free OVA model. However, this difference did not greatly alter the clinical outcome of the disease. This finding suggests that the role of NLRP3 in allergic asthma must be re-evaluated. The Journal of Immunology, 2012, 188: 2884-2893.
Details
- Title: Subtitle
- Analysis of NLRP3 in the Development of Allergic Airway Disease in Mice
- Creators
- Irving C. Allen - University of North Carolina at Chapel HillCorey M. Jania - University of North Carolina at Chapel HillJustin E. Wilson - University of North Carolina at Chapel HillErin M. Tekeppe - University of North Carolina at Chapel HillXiaoyang Hua - University of North Carolina at Chapel HillWillie J. Brickey - University of North Carolina at Chapel HillMildred Kwan - University of North Carolina at Chapel HillBeverly H. Koller - University of North Carolina at Chapel HillStephen L. Tilley - University of North Carolina at Chapel HillJenny P. -Y. Ting - University of North Carolina at Chapel Hill
- Resource Type
- Journal article
- Publication Details
- The Journal of immunology (1950), Vol.188(6), pp.2884-2893
- DOI
- 10.4049/jimmunol.1102488
- PMID
- 22323538
- PMCID
- PMC3294123
- NLM abbreviation
- J Immunol
- ISSN
- 0022-1767
- eISSN
- 1550-6606
- Publisher
- Amer Assoc Immunologists
- Number of pages
- 10
- Grant note
- A1063031; U19-AI067798; U19-AI077437; DK38108; DE016326; HL068141; F32-AI-082895-01; T32-AR007416; T32-CA009156 / National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA K01DK092355 / NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) R01DE016326 / NATIONAL INSTITUTE OF DENTAL &CRANIOFACIAL RESEARCH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR) T32CA009156 / NATIONAL CANCER INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) T32HL083828 / NATIONAL HEART, LUNG, AND BLOOD INSTITUTE; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Heart Lung & Blood Institute (NHLBI) F32AI082895 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) Sandler Program Award T32AR007416 / NATIONAL INSTITUTE OF ARTHRITIS AND MUSCULOSKELETAL AND SKIN DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Arthritis & Musculoskeletal & Skin Diseases (NIAMS) American Cancer Society
- Language
- English
- Date published
- 03/15/2012
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984311437202771
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