Analysis of Nevirapine Resistance Mutations in Cloned HIV Type 1 Variants from HIV-Infected Ugandan Infants Using a Single-Step Amplification-Sequencing Method (AmpliSeq)

William Ian TOWLER; Jessica D CHURCH; James R ESHLEMAN; Mary Glenn FOWLER; Laura A GUAY; J. Brooks JACKSON; Susan H ESHLEMAN

doi:10.1089/aid.2008.0109

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Analysis of Nevirapine Resistance Mutations in Cloned HIV Type 1 Variants from HIV-Infected Ugandan Infants Using a Single-Step Amplification-Sequencing Method (AmpliSeq)

Journal article

Open access

Peer reviewed

Analysis of Nevirapine Resistance Mutations in Cloned HIV Type 1 Variants from HIV-Infected Ugandan Infants Using a Single-Step Amplification-Sequencing Method (AmpliSeq)

William Ian TOWLER, Jessica D CHURCH, James R ESHLEMAN, Mary Glenn FOWLER, Laura A GUAY, J. Brooks JACKSON and Susan H ESHLEMAN

AIDS research and human retroviruses, Vol.24(9), pp.1209-1213

2008

DOI: 10.1089/aid.2008.0109

PMCID: PMC2562759

PMID: 18788912

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Abstract

We analyzed the genetic linkage of nevirapine (NVP) resistance mutations and the genetic complexity of HIV-1 variants in Ugandan infants who were HIV infected despite single dose (SD) prophylaxis. Plasma samples were obtained from six HIV-infected infants who had two or more NVP resistance mutations detected by population sequencing (ViroSeq). ViroSeq PCR products were cloned and transformed, and a single-step amplification-sequencing reaction (AmpliSeq) was used to analyze NVP resistance mutations in cloned HIV-1 variants directly from bacterial colonies. Fifty clones were analyzed for each infant sample. This analysis revealed numerous NVP resistance mutations not detected by population sequencing, genetically linked NVP resistance mutations, and a high degree of genetic complexity at codons that influence NVP susceptibility.

Infectious Diseases

Microbiology

Virology

Fundamental and applied biological sciences. Psychology

Action of physical and chemical agents

Miscellaneous

Viral diseases

Viral diseases of the lymphoid tissue and the blood. Aids

Biological and medical sciences

Medical sciences

Human viral diseases

Details

Title: Subtitle: Analysis of Nevirapine Resistance Mutations in Cloned HIV Type 1 Variants from HIV-Infected Ugandan Infants Using a Single-Step Amplification-Sequencing Method (AmpliSeq)
Creators: William Ian TOWLER - Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, United States
Jessica D CHURCH - Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, United States
James R ESHLEMAN - Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, United States
Mary Glenn FOWLER - Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, United States
Laura A GUAY - Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, United States
J. Brooks JACKSON - Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, United States
Susan H ESHLEMAN - Department of Pathology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, United States
Resource Type: Journal article
Publication Details: AIDS research and human retroviruses, Vol.24(9), pp.1209-1213
DOI: 10.1089/aid.2008.0109
PMID: 18788912
PMCID: PMC2562759
NLM abbreviation: AIDS Res Hum Retroviruses
ISSN: 0889-2229
eISSN: 1931-8405
Publisher: Liebert; Larchmont, NY
Language: English
Date published: 2008
Academic Unit: Pathology; VPMA - Administration
Record Identifier: 9984047621002771

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