Journal article
Analysis of Xenopus laevis ovary and somatic cell polyadenylated RNA by molecular hybridization
Developmental Biology, Vol.63(1), pp.197-212
1978
DOI: 10.1016/0012-1606(78)90125-2
PMID: 564793
Abstract
The number of sequences represented in the polyadenylated RNA population of the Xenopus oocyte and the degree to which these sequences are specific for early development have been determined by RNA-cDNA hybridization. Approximately 20,000 different sequences are found in the immature oocyte and the mature oocyte, and these sequences are the same in both types of cells. Approximately 5% of these sequences are present at a 15-fold higher concentration than the other 95%. The percentage of nonrepeated nuclear DNA homologous to the polyadenylated RNA was also determined and was in agreement with the number of sequences determined by the cDNA measurement. In addition, the number of sequences present in the Xenopus laevis kidney tissue culture cell and in the swimming tadpole was measured; approximately 20,000 different sequences were present in both the tissue culture cell and in the tadpole. The majority of the sequences present in the oocyte are not specific for early development since they are shared with both the tissue culture cell and the swimming tadpole; however, some sequences appear to be greatly increased in relative abundance in the tissue culture cell and the tadpole as compared to the oocyte.
Details
- Title: Subtitle
- Analysis of Xenopus laevis ovary and somatic cell polyadenylated RNA by molecular hybridization
- Creators
- S Perlman - Institute of Animal Genetics, University of Edinburgh, Edinburgh, ScotlandM Rosbash - Department of Biology and Rosenstiel Basic Medical Sciences Research Center, Brandeis University, Waltham, Massachusetts USA
- Resource Type
- Journal article
- Publication Details
- Developmental Biology, Vol.63(1), pp.197-212
- DOI
- 10.1016/0012-1606(78)90125-2
- PMID
- 564793
- NLM abbreviation
- Dev Biol
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Publisher
- Elsevier Inc
- Language
- English
- Date published
- 1978
- Academic Unit
- Microbiology and Immunology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Infectious Disease (Pediatrics)
- Record Identifier
- 9983777351902771
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