Journal article
Analysis of a non-structural gene reveals evidence of possible hepatitis C virus (HCV) compartmentalization
Journal of medical virology, Vol.84(2), pp.242-252
02/2012
DOI: 10.1002/jmv.22269
PMCID: PMC3243959
PMID: 22170544
Abstract
Viral diversity is a hallmark of hepatitis C virus (HCV) infection; however, only limited data are available regarding HCV variability in extrahepatic sites, and none have systematically compared diversity in non-structural and structural genomic regions. Therefore, HCV diversity in the NS5B and envelope 1 (E1) hypervariable region 1 (HVR1) genes was evaluated in matched sera and peripheral blood mononuclear cells (PBMCs) obtained from 13 HCV-infected women. Multiple clonal sequences were compared to evaluate quasispecies diversity and viral compartmentalization in PBMCs.
Genetic distances were higher for E1/HVR1 compared to NS5B in both the sera and PBMCs (p = 0.0511 and p = 0.0284). Genetic distances were higher in serum NS5B compared to PBMC NS5B (p = 0.0003); however, they were not different when comparing E1/HVR1 in sera to PBMCs. By phylogenetic analysis of NS5B, evidence of possible PBMC compartmentalization was observed for 1 woman, while statistical methods were consistent with PBMC compartmentalization for 6 women. Evidence of compartmentalization within a non-structural genomic region may suggest that viral adaptation to a unique extracellular microenvironment(s) may be required for efficient replication and could contribute to HCV persistence.
Details
- Title: Subtitle
- Analysis of a non-structural gene reveals evidence of possible hepatitis C virus (HCV) compartmentalization
- Creators
- Jason T. Blackard - University of Cincinnati Medical CenterGang Ma - University of Cincinnati Medical CenterJeffrey A. Welge - University of Cincinnati Medical CenterChristina M. Martin - University of Cincinnati Medical CenterKenneth E. Sherman - University of Cincinnati Medical CenterLynn E. Taylor - Miriam HospitalKenneth H. Mayer - Miriam HospitalDenise J. Jamieson - Centers for Disease Control and Prevention
- Resource Type
- Journal article
- Publication Details
- Journal of medical virology, Vol.84(2), pp.242-252
- DOI
- 10.1002/jmv.22269
- PMID
- 22170544
- PMCID
- PMC3243959
- ISSN
- 0146-6615
- eISSN
- 1096-9071
- Grant note
- R21 DA022148-02 || DA / National Institute on Drug Abuse : NIDA
- Language
- English
- Date published
- 02/2012
- Academic Unit
- Obstetrics and Gynecology; VPMA - Administration
- Record Identifier
- 9984446517102771
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