Journal article
Analysis of a primer‐independent GTF‐I from Streptococcus salivarius
FEMS microbiology letters, Vol.123(3), pp.349-354
11/1994
DOI: 10.1111/j.1574-6968.1994.tb07247.x
PMID: 7988916
Abstract
A glucosyltransferase (GTF) gene, designated gtfL, from Streptococcus salivarius was cloned and expressed in Escherichia coli and its nucleotide sequence determined. The GTF‐L enzyme catalysed the synthesis of water‐insoluble glucan in a primer‐independent manner. The nucleotide sequence and derived amino acid sequence of GTF‐L were similar in size and domain structure to previously sequenced glucosyltransferases. However, a 464‐bp region of high variability was identified which could be selectively amplified from strains of S. salivarius by the polymerase chain reaction and could therefore form the basis for species identification. No sequence‐specific motifs related to the solubility and linkage of the glucan product or its need for a dextran primer could be ascertained.
Details
- Title: Subtitle
- Analysis of a primer‐independent GTF‐I from Streptococcus salivarius
- Creators
- Jeffrey A. Banas - University of OklahomaDaniel Simon - University of OklahomaLisa K. WilliamsJoseph J. Ferretti - University of OklahomaRoy R.B. Russell - University of Newcastle Australia
- Resource Type
- Journal article
- Publication Details
- FEMS microbiology letters, Vol.123(3), pp.349-354
- Publisher
- Blackwell Publishing Ltd
- DOI
- 10.1111/j.1574-6968.1994.tb07247.x
- PMID
- 7988916
- ISSN
- 0378-1097
- eISSN
- 1574-6968
- Number of pages
- 6
- Language
- English
- Date published
- 11/1994
- Academic Unit
- Pediatric Dentistry; Dental Research
- Record Identifier
- 9984367640002771
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