Analysis of insertions and deletions in the gag p6 region of diverse HIV type 1 strains

Natalia MARLOWE; Tamara FLYS; John JR HACKETT; Michael SCHUMAKER; J. Brooks JACKSON; Susan H ESHLEMAN

doi:10.1089/aid.2004.20.1119

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Analysis of insertions and deletions in the gag p6 region of diverse HIV type 1 strains

Journal article

Peer reviewed

Analysis of insertions and deletions in the gag p6 region of diverse HIV type 1 strains

Natalia MARLOWE, Tamara FLYS, John JR HACKETT, Michael SCHUMAKER, J. Brooks JACKSON and Susan H ESHLEMAN

AIDS research and human retroviruses, Vol.20(10), pp.1119-1125

2004

DOI: 10.1089/aid.2004.20.1119

PMID: 15585104

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Abstract

Sequence variation in the gag p6 region in subtype B HIV-1 has been associated with changes in viral replication capacity and antiretroviral drug susceptibility. We examined sequence variation in the HIV-1 gag p6 region using plasma samples from 22 individuals with non-subtype B HIV-1 infection [subtypes A, C, D, F, and G, and circulating recombinant forms (CRFs) CRF01-AE and CRF02_AG]. An additional 105 gag sequences from the Los Alamos National Laboratory database were also analyzed. Extensive length variation was observed in the p6 gag region. Specific patterns of insertions and deletions were observed in different subtypes and CRFs, and no two subtypes or CRFs had the same general pattern. PTAP duplications were more common in subtype C than other strains (3 of 14 in subtype C vs. 2 of 113 in other strains, p = 0.004), and KQE duplications were seen only in subtype B. Further studies are needed to determine whether such genotypic differences influence viral replication capacity, antiretroviral drug susceptibility, or other phenotypic properties of these strains.

Infectious Diseases

Microbiology

Virology

Fundamental and applied biological sciences. Psychology

Miscellaneous

Viral diseases

Viral diseases of the lymphoid tissue and the blood. Aids

Biological and medical sciences

Medical sciences

Human viral diseases

Details

Title: Subtitle: Analysis of insertions and deletions in the gag p6 region of diverse HIV type 1 strains
Creators: Natalia MARLOWE - Celera Diagnostics, Alameda, California 94502, United States
Tamara FLYS - Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, United States
John JR HACKETT - AIDS Research and Retrovirus Discovery, Abbott Laboratories, Abbott Park, Illinois 60064, United States
Michael SCHUMAKER - Celera Diagnostics, Alameda, California 94502, United States
J. Brooks JACKSON - Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, United States
Susan H ESHLEMAN - Department of Pathology, The Johns Hopkins Medical Institutions, Baltimore, Maryland 21205, United States
Resource Type: Journal article
Publication Details: AIDS research and human retroviruses, Vol.20(10), pp.1119-1125
Publisher: Liebert; Larchmont, NY
DOI: 10.1089/aid.2004.20.1119
PMID: 15585104
ISSN: 0889-2229
eISSN: 1931-8405
Language: English
Date published: 2004
Academic Unit: Pathology; VPMA - Administration
Record Identifier: 9984046908702771

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