Journal article
Analysis of ligand dependence and hormone response element synergy in transcription by estrogen receptor
The Journal of steroid biochemistry and molecular biology, Vol.60(5), pp.285-294
1997
DOI: 10.1016/S0960-0760(96)00198-7
PMID: 9219919
Abstract
In this work we examined two questions: (1) Is the low, but readily detectable, ability of estrogen receptor (ER) to activate transcription in the absence of added 17β-estradiol caused by traces of estrogen in the growth medium, or by a weak ligand-independent ability of ER to activate transcription? (2) Does the ER exhibit synergistic activation of transcription on reporter genes containing multiple estrogen response elements (EREs)? To study these questions we developed a powerful new reporter gene, containing four EREs, which achieves inductions of up to 330-fold in the presence of liganded ER. We provided several types of evidence indicating that under standard cell culture conditions unliganded ER is unable to activate transcription. We demonstrated that when cells are grown in serum-free medium, estrogenic compounds may be in the base tissue culture medium. We demonstrated a strong cell and ER-dependence in transcriptional synergy, and suggest that cooperative binding of ER to multiple EREs can be responsible for transcriptional synergy
in vivo.
Details
- Title: Subtitle
- Analysis of ligand dependence and hormone response element synergy in transcription by estrogen receptor
- Creators
- Sandra MattickKevin GlennGeorgius de HaanDavid J Shapiro
- Resource Type
- Journal article
- Publication Details
- The Journal of steroid biochemistry and molecular biology, Vol.60(5), pp.285-294
- Publisher
- Elsevier Ltd
- DOI
- 10.1016/S0960-0760(96)00198-7
- PMID
- 9219919
- ISSN
- 0960-0760
- eISSN
- 1879-1220
- Language
- English
- Date published
- 1997
- Academic Unit
- General Internal Medicine; Internal Medicine
- Record Identifier
- 9984094592902771
Metrics
19 Record Views