Journal article
Analysis of nontypeable haemophilus influenzae phase-variable genes during experimental human nasopharyngeal colonization
The Journal of infectious diseases, Vol.208(5), pp.720-727
09/01/2013
DOI: 10.1093/infdis/jit240
PMCID: PMC3733508
PMID: 23715658
Abstract
Studies of nontypeable Haemophilus influenzae (NTHi) have demonstrated that a number of genes associated with infectivity have long repeat regions associated with phase variation in expression of the respective gene. The purpose of this study was to determine the genes that underwent phase variation during a 6-day period of experimental human nasopharyngeal colonization.
Strain NTHi 2019Str(R)1 was used to colonize the nasopharynx of human subjects in a study of experimental colonization. Thirteen phase-variable genes were analyzed in NTHi 2019Str(R)1. Samples of NTHi 2019Str(R)1 were cultured from subjects during the 6-day colonization period. We used capillary electrophoresis and Roche 454 pyrosequencing to determine the number of repeats in each gene from each sample.
A significant number of samples switched licA and igaB from phase off in the inoculated strain to phase on during the 4-day period of observation. lex2A also showed variability as compared to baseline, but the differences were not significant. The remaining genes showed no evidence of phase variation.
Our studies suggest that the phase-on genotypes of licA and igaB are important for early human nasopharynx colonization. lex2A showed a trend from phase off to phase on, suggesting a potentially important role in the colonization process.
Details
- Title: Subtitle
- Analysis of nontypeable haemophilus influenzae phase-variable genes during experimental human nasopharyngeal colonization
- Creators
- Jessica Poole - Department of Microbiology, University of Iowa, Iowa City, IA 52242, USAEric FosterKathryn ChalonerJason HuntMichael P JenningsThomas BairKevin KnudtsonErik ChristensenRobert S Munson JrPatricia L WinokurMichael A Apicella
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.208(5), pp.720-727
- DOI
- 10.1093/infdis/jit240
- PMID
- 23715658
- PMCID
- PMC3733508
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Grant note
- P30 ES005605 / NIEHS NIH HHS AI024616 / NIAID NIH HHS R01 AI024616 / NIAID NIH HHS R37 AI024616 / NIAID NIH HHS R56 AI024616 / NIAID NIH HHS UL1 TR000442 / NCATS NIH HHS UL1 TR00442 / NCATS NIH HHS N01AI30040 / NIAID NIH HHS AI30040 / NIAID NIH HHS
- Language
- English
- Date published
- 09/01/2013
- Academic Unit
- Preventive and Community Dentistry; Microbiology and Immunology; Infectious Diseases; College of Public Health; Medicine Administration; Internal Medicine; Iowa Institute of Human Genetics
- Record Identifier
- 9984094625402771
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