Journal article
Analysis of second-site mutations that suppress the multiple drug resistance phenotype of the yeast PDR1-7 allele
Gene, Vol.167(1), pp.151-155
1995
DOI: 10.1016/0378-1119(95)00663-X
PMID: 8566768
Abstract
The yeast
PDR1 locus encodes a member of the C6 zinc cluster family of transcriptional regulatory proteins. Among the targets of
PDR1 is the yeast
PDR5 locus. The product of this gene is a member of the ATP-binding cassette (ABC) transmembrane protein family and plays a major role in inhibitor efflux. Mutations in
PDR1 affect the relative level of
PDR5 transcript and can therefore result in increased or decreased drug resistance. We isolated three second-site suppressors of
PDR1-7 semidominant hyper-resistant mutation. These mutants were drug hypersensitive, as compared with isogenic controls. Two of the three mutations contained alterations in a putative DNA-binding domain. Significantly, the mutant proteins exhibited reduced DNA-binding capacity.
Details
- Title: Subtitle
- Analysis of second-site mutations that suppress the multiple drug resistance phenotype of the yeast PDR1-7 allele
- Creators
- Thomas M. McGuire - University of AmericaElvira Carvajal - Université Catholique de LouvainDavid Katzmann - University of IowaMarisa Wagner - Department of Biology, The Catholic University of America, Washington, DC 20064, USA.W.Scott Moye-Rowley - University of IowaAndré Goffeau - Université Catholique de LouvainJohn Golin - Department of Biology, The Catholic University of America, Washington, DC 20064, USA.
- Resource Type
- Journal article
- Publication Details
- Gene, Vol.167(1), pp.151-155
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0378-1119(95)00663-X
- PMID
- 8566768
- ISSN
- 0378-1119
- eISSN
- 1879-0038
- Language
- English
- Date published
- 1995
- Academic Unit
- Molecular Physiology and Biophysics; Internal Medicine
- Record Identifier
- 9984297499902771
Metrics
53 Record Views