Analysis of toxic shock syndrome isolates producing staphylococcal enterotoxins B and C1 with use of southern hybridization and immunologic assays

Gregory A Bohach; Barry N Kreiswirth; Richard P Novick; Patrick M Schlievert

doi:10.1093/clinids/11.Supplement_1.S75

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Journal article

Analysis of toxic shock syndrome isolates producing staphylococcal enterotoxins B and C1 with use of southern hybridization and immunologic assays

Gregory A Bohach, Barry N Kreiswirth, Richard P Novick and Patrick M Schlievert

Reviews of infectious diseases, Vol.11 Suppl 1, pp.S75-S82

01/1989

DOI: 10.1093/clinids/11.Supplement_1.S75

PMID: 2494691

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Abstract

A study was undertaken to evaluate the production of enterotoxin B (Ent B), EntC1, and toxic shock syndrome toxin 1 (TSST-1) by isolates of Staphylococcus aureus from patients with toxic shock syndrome (TSS) and from a variety of other sources. Levels of toxin in culture supernatants were measured by a quantitative immunodiffusion assay. Most vaginal TSS isolates produced TSST-1, either alone or with EntC1. However, strains that produced EntB or EntC1 but did not express TSST-1 were commonly isolated from patients with nonmenstrual TSS; EntB was usually produced alone or--rarely--with EntC1. These results were confirmed by probing DNA from representative isolates with an internal probe of the EntC1 gene (entC1). Extensive sequence homology between entC1 and entB enabled detection of both genes under conditions of high stringency. The genomic location of entC1 in strains producing both EntC1 and TSST-1 varied little but was dependent on the mammalian host. In contrast, the genomic location of entC1 or entB in strains producing EntC1 or EntB alone was variable. These results suggest that these genes are contained on mobile elements.

Nucleic Acid Hybridization

Staphylococcus aureus - genetics

Blotting, Southern

DNA Probes

Humans

Immunodiffusion

Molecular Sequence Data

Restriction Mapping

Sequence Homology, Nucleic Acid

Shock, Septic - microbiology

Enterotoxins - biosynthesis

Enterotoxins - genetics

Animals

DNA, Bacterial - genetics

Bacterial Toxins

Base Sequence

Superantigens

Staphylococcus aureus - metabolism

Details

Title: Subtitle: Analysis of toxic shock syndrome isolates producing staphylococcal enterotoxins B and C1 with use of southern hybridization and immunologic assays
Creators: Gregory A Bohach - Department of Microbiology, Medical School, University of Minnesota, Minneapolis 55455
Barry N Kreiswirth
Richard P Novick
Patrick M Schlievert
Resource Type: Journal article
Publication Details: Reviews of infectious diseases, Vol.11 Suppl 1, pp.S75-S82
Publisher: United States
DOI: 10.1093/clinids/11.Supplement_1.S75
PMID: 2494691
ISSN: 0162-0886
Grant note: AI 22159 / NIAID NIH HHS
Language: English
Date published: 01/1989
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001156002771

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