Journal article
Analytical Pipeline for Discovery and Verification of Glycoproteins from Plasma-Derived Extracellular Vesicles as Breast Cancer Biomarkers
Analytical chemistry (Washington), Vol.90(10), pp.6307-6313
05/15/2018
DOI: 10.1021/acs.analchem.8b01090
PMID: 29629753
Abstract
Glycoproteins comprise more than half of current FDA-approved protein cancer markers, but the development of new glycoproteins as disease biomarkers has been stagnant. Here we present a pipeline to develop glycoproteins from extracellular vesicles (EVs) through integrating quantitative glycoproteomics with a novel reverse phase glycoprotein array and then apply it to identify novel biomarkers for breast cancer. EV glycoproteomics show promise in circumventing the problems plaguing current serum/plasma glycoproteomics and allowed us to identify hundreds of glycoproteins that have not been identified in blood. We identified 1,453 unique glycopeptides representing 556 glycoproteins in EVs, among which 20 were verified significantly higher in individual breast cancer patients. We further applied a novel glyco-specific reverse phase protein array to quantify a subset of the candidates. Together, this study demonstrates the great potential of this integrated pipeline for biomarker discovery.
Details
- Title: Subtitle
- Analytical Pipeline for Discovery and Verification of Glycoproteins from Plasma-Derived Extracellular Vesicles as Breast Cancer Biomarkers
- Creators
- I-Hsuan Chen - Purdue University West LafayetteHilary Andaluz Aguilar - Purdue University West LafayetteJ. Sebastian Paez Paez - Purdue University West LafayetteXiaofeng Wu - Purdue University West LafayetteLi Pan - Purdue University West LafayetteMichael K. Wendt - Purdue University West LafayetteAnton B. Iliuk - Tymora Analytical OperationsYing Zhang - Fudan University Shanghai Cancer CenterW. Andy Tao - Purdue University West Lafayette
- Resource Type
- Journal article
- Publication Details
- Analytical chemistry (Washington), Vol.90(10), pp.6307-6313
- DOI
- 10.1021/acs.analchem.8b01090
- PMID
- 29629753
- NLM abbreviation
- Anal Chem
- ISSN
- 0003-2700
- eISSN
- 1520-6882
- Publisher
- Amer Chemical Soc
- Number of pages
- 7
- Grant note
- 1506752 / NSF; National Science Foundation (NSF) P30 CA023168 / Purdue University Center for Cancer Research R01GM111788 / NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of General Medical Sciences (NIGMS) 5R01GM088317; 1R0IGM111788; S10 RR025044 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 05/15/2018
- Academic Unit
- Internal Medicine
- Record Identifier
- 9984459625302771
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