Androgen regulation of epidermal growth factor receptor binding activity during fetal rabbit lung development

J M Klein; H C Nielsen

doi:10.1172/JCI116218

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Androgen regulation of epidermal growth factor receptor binding activity during fetal rabbit lung development

Journal article

Open access

Peer reviewed

Androgen regulation of epidermal growth factor receptor binding activity during fetal rabbit lung development

J M Klein and H C Nielsen

The Journal of clinical investigation, Vol.91(2), pp.425-431

02/1993

DOI: 10.1172/JCI116218

PMCID: PMC287946

PMID: 8432851

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https://doi.org/10.1172/JCI116218View

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Abstract

Fetal lung development progresses in a sex-specific manner with male fetuses exhibiting delayed maturation. Androgens, both exogenous and endogenous, inhibit while epidermal growth factor (EGF) enhances fetal lung development. We hypothesized that one mechanism responsible for the delay in male fetal lung development is an androgen-induced delay in EGF receptor binding activity. We measured EGF binding in sex-specific fetal rabbit lung plasma membranes isolated from control fetuses (days 21, 23, 25, 27, 29, and 30 of gestation) and from androgen-treated fetuses (days 21, 23, and 27 of gestation) that had been continuously exposed in vivo to exogenous 5 alpha-dihydrotestosterone from day 12 through 27 of gestation. Specific binding of EGF was significantly lower in male than in female fetal lung tissue isolated from controls at day 21 of gestation. Scatchard analysis revealed that this decrease in EGF binding was associated with decreased EGF receptor density without any significant change in affinity. Prenatal exogenous androgen treatment led to decreased EGF binding in fetal rabbit lung tissue from both sexes secondary to a decrease in EGF receptor density. These findings suggest that one mechanism responsible for the delay in male fetal lung maturation is an androgen-induced delay in EGF receptor binding activity during fetal lung development.

Binding, Competitive

Fetal Organ Maturity - physiology

Rabbits

Phosphorylation

Androgens - physiology

Epidermal Growth Factor - metabolism

Male

Pregnancy

Receptor, Epidermal Growth Factor - metabolism

Animals

Androgens - pharmacology

Sex Factors

Female

Lung - embryology

Lung - metabolism

Dihydrotestosterone - metabolism

Details

Title: Subtitle: Androgen regulation of epidermal growth factor receptor binding activity during fetal rabbit lung development
Creators: J M Klein - Department of Pediatrics, University of Iowa, Iowa City 52242
H C Nielsen
Resource Type: Journal article
Publication Details: The Journal of clinical investigation, Vol.91(2), pp.425-431
DOI: 10.1172/JCI116218
PMID: 8432851
PMCID: PMC287946
ISSN: 0021-9738
eISSN: 1558-8238
Grant note: R01 HL43407 / NHLBI NIH HHS P30 HD27748 / NICHD NIH HHS R01 HL37930 / NHLBI NIH HHS R01 HL037930 / NHLBI NIH HHS
Language: English
Date published: 02/1993
Academic Unit: Stead Family Department of Pediatrics; Neonatology
Record Identifier: 9984093356702771

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