Journal article
Angiotensin Selectively Activates a Subpopulation of Postganglionic Sympathetic Neurons in Mice
Circulation research, Vol.88(8), pp.787-793
04/27/2001
DOI: 10.1161/hh0801.089542
PMID: 11325870
Abstract
ABSTRACT—Angiotensin II (Ang II) increases renal sympathetic nerve activity in anesthetized mice before and after ganglionic blockade, suggesting that Ang II may directly activate postganglionic sympathetic neurons. The present study directly tested this hypothesis in vitro. Neurons were dissociated from aortic-renal and celiac ganglia of C57BL/6J mice. Cytosolic Ca concentration ([Ca]i) was measured with ratio imaging using fura 2. Ang II increased [Ca]i in a subpopulation of sympathetic neurons. At a concentration of 200 nmol/L, 14 (67%) of 21 neurons responded with a rise in [Ca]i. The Ang II type 1 (AT1) receptor blocker (losartan, 2 μmol/L) but not the Ang II type 2 (AT2) receptor blocker (PD123,319, 4 μmol/L) blocked this effect. The Ang II–induced [Ca]i increase was abolished by removal of extracellular Ca but not altered by depletion of intracellular Ca stores with thapsigargin. Ang II no longer elicited a [Ca]i increase in the presence of lanthanum (25 μmol/L). The specific N-type and L-type Ca channel blockers, ω-conotoxin GVIA and nifedipine, respectively, significantly inhibited the Ang II–induced [Ca]i increase. The protein kinase C inhibitor H7 but not the protein kinase A inhibitor H89 blocked the response to Ang II. These results demonstrate that Ang II selectively activates a subpopulation of postganglionic sympathetic neurons in aortic-renal and celiac ganglia, triggering Ca influx through voltage-gated Ca channels. This effect is mediated through AT1 receptors and requires the activation of protein kinase C. The activation of a subgroup of sympathetic neurons by Ang II may exert unique effects on kidney function in pathological states associated with elevated Ang II.
Details
- Title: Subtitle
- Angiotensin Selectively Activates a Subpopulation of Postganglionic Sympathetic Neurons in Mice
- Creators
- Xiuying Ma - From the Departments of Internal Medicine (X.Y.M., M.W.C., C.A.W., F.M.A., K.B.) and Physiology and Biophysics (F.M.A.), The Cardiovascular Center, University of Iowa College of Medicine, and the Department of Veterans Affairs Medical Center (M.W.C.), Iowa City, IowaMark ChapleauCarol WhiteisFrancois AbboudKlaus Bielefeldt
- Resource Type
- Journal article
- Publication Details
- Circulation research, Vol.88(8), pp.787-793
- Publisher
- American Heart Association, Inc
- DOI
- 10.1161/hh0801.089542
- PMID
- 11325870
- ISSN
- 0009-7330
- eISSN
- 1524-4571
- Language
- English
- Date published
- 04/27/2001
- Academic Unit
- Molecular Physiology and Biophysics; Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984025593202771
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