Animal and Human Multidrug-Resistant, Cephalosporin-Resistant Salmonella Isolates Expressing a Plasmid-Mediated CMY-2 AmpC β-Lactamase

P. L Winokur; A Brueggemann; D. L DeSalvo; L Hoffmann; M. D Apley; E. K Uhlenhopp; M. A Pfaller; G. V Doern

doi:10.1128/AAC.44.10.2777-2783.2000

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Animal and Human Multidrug-Resistant, Cephalosporin-Resistant Salmonella Isolates Expressing a Plasmid-Mediated CMY-2 AmpC β-Lactamase

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Animal and Human Multidrug-Resistant, Cephalosporin-Resistant Salmonella Isolates Expressing a Plasmid-Mediated CMY-2 AmpC β-Lactamase

P. L Winokur, A Brueggemann, D. L DeSalvo, L Hoffmann, M. D Apley, E. K Uhlenhopp, M. A Pfaller and G. V Doern

Antimicrobial agents and chemotherapy, Vol.44(10), pp.2777-2783

10/2000

DOI: 10.1128/AAC.44.10.2777-2783.2000

PMCID: PMC90151

PMID: 10991860

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Abstract

Salmonella spp. are important food-borne pathogens that are demonstrating increasing antimicrobial resistance rates in isolates obtained from food animals and humans. In this study, 10 multidrug-resistant, cephalosporin-resistant Salmonella isolates from bovine, porcine, and human sources from a single geographic region were identified. All isolates demonstrated resistance to cephamycins and extended-spectrum cephalosporins as well as tetracycline, chloramphenicol, streptomycin, and sulfisoxazole. Molecular epidemiological analyses revealed eight distinct chromosomal DNA patterns, suggesting that clonal spread could not entirely explain the distribution of this antimicrobial resistance phenotype. However, all isolates encoded an AmpC-like β-lactamase, CMY-2. Eight isolates contained a large nonconjugative plasmid that could transform Escherichia coli . Transformants coexpressed cephalosporin, tetracycline, chloramphenicol, streptomycin, and sulfisoxazole resistances. Plasmid DNA revealed highly related restriction fragments though plasmids appeared to have undergone some evolution over time. Multidrug-resistant, cephalosporin-resistant Salmonella spp. present significant therapeutic problems in animal and human health care and raise further questions about the association between antimicrobial resistance, antibiotic use in animals, and transfer of multidrug-resistant Salmonella spp. between animals and man.

Mechanisms of Resistance

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Title: Subtitle: Animal and Human Multidrug-Resistant, Cephalosporin-Resistant Salmonella Isolates Expressing a Plasmid-Mediated CMY-2 AmpC β-Lactamase
Creators: P. L Winokur - University of Iowa College of Medicine
A Brueggemann - University of Iowa College of Medicine
D. L DeSalvo - University of Iowa College of Medicine
L Hoffmann - University of Iowa College of Medicine
M. D Apley - University of Iowa College of Medicine
E. K Uhlenhopp - University of Iowa College of Medicine
M. A Pfaller - University of Iowa College of Medicine
G. V Doern - University of Iowa College of Medicine
Resource Type: Journal article
Publication Details: Antimicrobial agents and chemotherapy, Vol.44(10), pp.2777-2783
DOI: 10.1128/AAC.44.10.2777-2783.2000
PMID: 10991860
PMCID: PMC90151
NLM abbreviation: Antimicrob Agents Chemother
ISSN: 0066-4804
eISSN: 1098-6596
Publisher: American Society for Microbiology
Language: English
Date published: 10/2000
Academic Unit: Microbiology and Immunology; Infectious Diseases; Epidemiology; Pathology; Medicine Administration; Internal Medicine
Record Identifier: 9984094600702771

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