Journal article
Anion Transport Across Human Gallbladder Organoids and Monolayers
Frontiers in physiology, Vol.13, pp.882525-882525
2022
DOI: 10.3389/fphys.2022.882525
PMCID: PMC9171199
PMID: 35685290
Abstract
Fluid and anion secretion are important functions of the biliary tract. It has been established that cAMP regulates Na
absorption through NHE3. However, mechanisms of gallbladder anion transport are less defined. We created organoids and organoid-derived monolayers from human gallbladder tissue to measure organoid swelling and transepithelial electrophysiology. In our
models, forskolin-stimulation caused organoid swelling and increased transepithelial anion transport. Full organoid swelling required Cl
while changes in short-circuit current were HCO
-dependent. Organoids and monolayers from an individual homozygous for the cystic fibrosis-causing
mutation had no apical expression of CFTR and minimal changes in transepithelial current and conductance with forskolin treatment. However, organoid swelling remained intact. Dilution potential studies revealed that forskolin treatment increased the paracellular permeability to anions relative to cations. These data suggest a novel paracellular contribution to forskolin-stimulated fluid transport across the gallbladder epithelium.
Details
- Title: Subtitle
- Anion Transport Across Human Gallbladder Organoids and Monolayers
- Creators
- Keyan Zarei - University of IowaIan M Thornell - University of IowaDavid A Stoltz - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Frontiers in physiology, Vol.13, pp.882525-882525
- DOI
- 10.3389/fphys.2022.882525
- PMID
- 35685290
- PMCID
- PMC9171199
- NLM abbreviation
- Front Physiol
- ISSN
- 1664-042X
- eISSN
- 1664-042X
- Grant note
- DOI: 10.13039/100000050, name: National Heart, Lung, and Blood Institute, award: 091842 007638; DOI: 10.13039/100000057, name: National Institute of General Medical Sciences, award: 007337; DOI: 10.13039/100000897, name: Cystic Fibrosis Foundation, award: STOLTZ19R0; DOI: 10.13039/100017784, name: Gilead Research Scholars; DOI: 10.13039/100000002, name: National Institutes of Health, award: CA0860862
- Language
- English
- Date published
- 2022
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984297496902771
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