Ankyrin-G Promotes Cyclic Nucleotide-Gated Channel Transport to Rod Photoreceptor Sensory Cilia

Krishnakumar KIZHATIL; Sheila A BAKER; Vadim Y ARSHAVSKY; Vann BENNETT

doi:10.1126/science.1169789

Back

Ankyrin-G Promotes Cyclic Nucleotide-Gated Channel Transport to Rod Photoreceptor Sensory Cilia

Journal article

Open access

Peer reviewed

Ankyrin-G Promotes Cyclic Nucleotide-Gated Channel Transport to Rod Photoreceptor Sensory Cilia

Krishnakumar KIZHATIL, Sheila A BAKER, Vadim Y ARSHAVSKY and Vann BENNETT

Science (American Association for the Advancement of Science), Vol.323(5921), pp.1614-1617

2009

DOI: 10.1126/science.1169789

PMID: 19299621

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/2792576View

Open Access

Abstract

Cyclic nucleotide-gated (CNG) channels localize exclusively to the plasma membrane of photosensitive outer segments of rod photoreceptors where they generate the electrical response to light. Here, we report the finding that targeting of CNG channels to the rod outer segment required their interaction with ankyrin-G. Ankyrin-G localized exclusively to rod outer segments, coimmunoprecipitated with the CNG channel, and bound to the C-terminal domain of the channel beta1 subunit. Ankyrin-G depletion in neonatal mouse retinas markedly reduced CNG channel expression. Transgenic expression of CNG channel beta-subunit mutants in Xenopus rods showed that ankyrin-G binding was necessary and sufficient for targeting of the beta1 subunit to outer segments. Thus, ankyrin-G is required for transport of CNG channels to the plasma membrane of rod outer segments.

Fundamental and applied biological sciences. Psychology

Vertebrates: nervous system and sense organs

Biological and medical sciences

Eye and associated structures. Visual pathways and centers. Vision

Details

Title: Subtitle: Ankyrin-G Promotes Cyclic Nucleotide-Gated Channel Transport to Rod Photoreceptor Sensory Cilia
Creators: Krishnakumar KIZHATIL - Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, United States
Sheila A BAKER - Department of Ophthalmology, Duke University Medical Center, Durham, NC 27710, United States
Vadim Y ARSHAVSKY - Department of Ophthalmology, Duke University Medical Center, Durham, NC 27710, United States
Vann BENNETT - Department of Cell Biology, Duke University Medical Center, Durham, NC 27710, United States
Resource Type: Journal article
Publication Details: Science (American Association for the Advancement of Science), Vol.323(5921), pp.1614-1617
DOI: 10.1126/science.1169789
PMID: 19299621
NLM abbreviation: Science
ISSN: 0036-8075
eISSN: 1095-9203
Publisher: American Association for the Advancement of Science; Washington, DC
Language: English
Date published: 2009
Academic Unit: Iowa Neuroscience Institute; Biochemistry and Molecular Biology; University College Courses; Ophthalmology and Visual Sciences
Record Identifier: 9984024504702771

Metrics

28 Record Views

60 Times Cited - Web of Science