Ankyrin G organizes membrane components to promote coupling of cell mechanics and glucose uptake

Alicia M Salvi; Jennifer L Bays; Samantha R Mackin; René-Marc Mege; Kris A DeMali

doi:10.1038/s41556-021-00677-y

Back

Ankyrin G organizes membrane components to promote coupling of cell mechanics and glucose uptake

Journal article

Peer reviewed

Ankyrin G organizes membrane components to promote coupling of cell mechanics and glucose uptake

Alicia M Salvi, Jennifer L Bays, Samantha R Mackin, René-Marc Mege and Kris A DeMali

Nature cell biology, Vol.23(5), pp.457-466

05/2021

DOI: 10.1038/s41556-021-00677-y

PMCID: PMC8428240

PMID: 33972734

Files and links (1)

url

https://hal.science/hal-03858079View

Open Access

Abstract

The response of cells to forces is critical for their function and occurs via rearrangement of the actin cytoskeleton . Cytoskeletal remodelling is energetically costly , yet how cells signal for nutrient uptake remains undefined. Here we present evidence that force transmission increases glucose uptake by stimulating glucose transporter 1 (GLUT1). GLUT1 recruitment to and retention at sites of force transmission requires non-muscle myosin IIA-mediated contractility and ankyrin G. Ankyrin G forms a bridge between the force-transducing receptors and GLUT1. This bridge is critical for enabling cells under tension to tune glucose uptake to support remodelling of the actin cytoskeleton and formation of an epithelial barrier. Collectively, these data reveal an unexpected mechanism for how cells under tension take up nutrients and provide insight into how defects in glucose transport and mechanics might be linked.

Ankyrins - metabolism

Biological Transport - physiology

Carrier Proteins - metabolism

Cell Membrane - metabolism

Cytoskeleton - metabolism

Glucose - metabolism

Glucose Transporter Type 1 - metabolism

Humans

Signal Transduction - physiology

Details

Title: Subtitle: Ankyrin G organizes membrane components to promote coupling of cell mechanics and glucose uptake
Creators: Alicia M Salvi - University of Iowa
Jennifer L Bays - Roy J. and Lucille A. Carver College of Medicine
Samantha R Mackin - University of Iowa
René-Marc Mege - Institut Jacques Monod
Kris A DeMali - Roy J. and Lucille A. Carver College of Medicine
Resource Type: Journal article
Publication Details: Nature cell biology, Vol.23(5), pp.457-466
DOI: 10.1038/s41556-021-00677-y
PMID: 33972734
PMCID: PMC8428240
NLM abbreviation: Nat Cell Biol
ISSN: 1465-7392
eISSN: 1476-4679
Grant note: T32 GM067795 / NIGMS NIH HHS R35 GM136291 / NIGMS NIH HHS P30 CA086862 / NCI NIH HHS F31 GM135962 / NIGMS NIH HHS
Language: English
Date published: 05/2021
Academic Unit: Dermatology; Microbiology and Immunology; Fraternal Order of Eagles Diabetes Research Center; Biochemistry and Molecular Biology
Record Identifier: 9984288721902771

Metrics

27 Record Views

23 Times Cited - Web of Science