Journal article
Anomalous incisor morphology indicates tissue-specific roles for Tfap2a and Tfap2b in tooth development
Developmental biology, Vol.472, pp.67-74
04/01/2021
DOI: 10.1016/j.ydbio.2020.12.017
PMCID: PMC8018193
PMID: 33460639
Abstract
Mice possess two types of teeth that differ in their cusp patterns; incisors have one cusp and molars have multiple cusps. The patterning of these two types of teeth relies on fine-tuning of the reciprocal molecular signaling between dental epithelial and mesenchymal tissues during embryonic development. The AP-2 transcription factors, particularly Tfap2a and Tfap2b, are essential components of such epithelial-mesenchymal signaling interactions that coordinate craniofacial development in mice and other vertebrates, but little is known about their roles in the regulation of tooth development and shape. Here we demonstrate that incisors and molars differ in their temporal and spatial expression of Tfap2a and Tfap2b. At the bud stage, Tfap2a is expressed in both the epithelium and mesenchyme of the incisors and molars, but Tfap2b expression is restricted to the molar mesenchyme, only later appearing in the incisor epithelium. Tissue-specific deletions show that loss of the epithelial domain of Tfap2a and Tfap2b affects the number and spatial arrangement of the incisors, notably resulting in duplicated lower incisors. In contrast, deletion of these two genes in the mesenchymal domain has little effect on tooth development. Collectively these results implicate epithelial expression of Tfap2a and Tfap2b in regulating the extent of the dental lamina associated with patterning the incisors and suggest that these genes contribute to morphological differences between anterior (incisor) and posterior (molar) teeth within the mammalian dentition.
Details
- Title: Subtitle
- Anomalous incisor morphology indicates tissue-specific roles for Tfap2a and Tfap2b in tooth development
- Creators
- Emily D. Woodruff - University of FloridaGalaxy C. Gutierrez - University of FloridaEric Van Otterloo - University of Colorado Anschutz Medical CampusTrevor Williams - University of Colorado Anschutz Medical CampusMartin J. Cohn - University of Florida
- Resource Type
- Journal article
- Publication Details
- Developmental biology, Vol.472, pp.67-74
- DOI
- 10.1016/j.ydbio.2020.12.017
- PMID
- 33460639
- PMCID
- PMC8018193
- NLM abbreviation
- Dev Biol
- ISSN
- 0012-1606
- eISSN
- 1095-564X
- Publisher
- Elsevier
- Number of pages
- 8
- Grant note
- DDRI 1455572 / NSF; National Science Foundation (NSF) 2R01 DE12728 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA American Society of Mammalogists K99/R00 DE026823 / NIDCR; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Dental & Craniofacial Research (NIDCR)
- Language
- English
- Date published
- 04/01/2021
- Academic Unit
- Anatomy and Cell Biology; Craniofacial Anomalies Research Center; Dental Research; Periodontics
- Record Identifier
- 9984284356502771
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