Anopheles gambiae pilot gene discovery project: identification of mosquito innate immunity genes from expressed sequence tags generated from immune-competent cell lines

George Dimopoulos; Thomas L Casavant; Shereen Chang; Todd  Scheetz; Chad  Roberts; Micca Donohue; Jörg Schultz; Vladimir Benes; Peer Bork; Wilhelm Ansorge; Marcelo Bento Soares; Fotis C Kafatos

doi:10.1073/pnas.97.12.6619

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Anopheles gambiae pilot gene discovery project: identification of mosquito innate immunity genes from expressed sequence tags generated from immune-competent cell lines

Journal article

Open access

Peer reviewed

Anopheles gambiae pilot gene discovery project: identification of mosquito innate immunity genes from expressed sequence tags generated from immune-competent cell lines

George Dimopoulos, Thomas L Casavant, Shereen Chang, Todd Scheetz, Chad Roberts, Micca Donohue, Jörg Schultz, Vladimir Benes, Peer Bork, Wilhelm Ansorge, …

Proceedings of the National Academy of Sciences - PNAS, Vol.97(12), pp.6619-6624

06/06/2000

DOI: 10.1073/pnas.97.12.6619

PMID: 10841561

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Abstract

Together with AIDS and tuberculosis, malaria is at the top of the list of devastating infectious diseases. However, molecular genetic studies of its major vector, Anopheles gambiae, are still quite limited. We have conducted a pilot gene discovery project to accelerate progress in the molecular analysis of vector biology, with emphasis on the mosquito's antimalarial immune defense. A total of 5,925 expressed sequence tags were determined from normalized cDNA libraries derived from immune-responsive hemocyte-like cell lines. The 3,242 expressed sequence tag-containing cDNA clones were grouped into 2,380 clone clusters, potentially representing unique genes. Of these, 1,118 showed similarities to known genes from other organisms, but only 27 were identical to previously known mosquito genes. We identified 38 candidate genes, based on sequence similarity, that may be implicated in immune reactions including antimalarial defense; 19 of these were shown experimentally to be inducible by bacterial challenge, lending support to their proposed involvement in mosquito immunity.

Cell Line

Multigene Family

Calcium-Binding Proteins

Collectins

Anopheles - genetics

Malaria - immunology

Molecular Sequence Data

Carrier Proteins - genetics

Animals

Insect Vectors - genetics

Base Sequence

Serine Endopeptidases - genetics

Malaria - transmission

Anopheles - immunology

Immunity - genetics

Periplasmic Binding Proteins

Monosaccharide Transport Proteins - genetics

Details

Title: Subtitle: Anopheles gambiae pilot gene discovery project: identification of mosquito innate immunity genes from expressed sequence tags generated from immune-competent cell lines
Creators: George Dimopoulos - European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany
Thomas L Casavant
Shereen Chang
Todd Scheetz
Chad Roberts
Micca Donohue
Jörg Schultz
Vladimir Benes
Peer Bork
Wilhelm Ansorge
Marcelo Bento Soares
Fotis C Kafatos
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.97(12), pp.6619-6624
DOI: 10.1073/pnas.97.12.6619
PMID: 10841561
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: United States
Language: English
Date published: 06/06/2000
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Electrical and Computer Engineering; Ophthalmology and Visual Sciences
Record Identifier: 9983980057802771

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