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Anterior chamber depth in mice is controlled by several quantitative trait loci
Journal article   Open access   Peer reviewed

Anterior chamber depth in mice is controlled by several quantitative trait loci

Demelza R. Larson, Allysa J. Kimber, Kacie J. Meyer and Michael G. Anderson
PloS one, Vol.18(8), e0286897
08/25/2023
DOI: 10.1371/journal.pone.0286897
PMCID: PMC10456175
PMID: 37624784
url
https://doi.org/10.1371/journal.pone.0286897View
Published (Version of record) Open Access

Abstract

Anterior chamber depth (ACD) is a quantitative trait associated with primary angle closure glaucoma (PACG). Although ACD is highly heritable, known genetic variations explain a small fraction of the phenotypic variability. The purpose of this study was to identify additional ACD-influencing loci using strains of mice. Cohorts of 86 N2 and 111 F2 mice were generated from crosses between recombinant inbred BXD24/TyJ and wild-derived CAST/EiJ mice. Using anterior chamber optical coherence tomography, mice were phenotyped at 10–12 weeks of age, genotyped based on 93 genome-wide SNPs, and subjected to quantitative trait locus (QTL) analysis. In an analysis of ACD among all mice, six loci passed the significance threshold of p = 0.05 and persisted after multiple regression analysis. These were on chromosomes 6, 7, 11, 12, 15 and 17 (named Acdq6 , Acdq7 , Acdq11 , Acdq12 , Acdq15 , and Acdq17 , respectively). Our findings demonstrate a quantitative multi-genic pattern of ACD inheritance in mice and identify six previously unrecognized ACD-influencing loci. We have taken a unique approach to studying the anterior chamber depth phenotype by using mice as genetic tool to examine this continuously distributed trait.

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