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Anti-Cryptococcus Phenalenones and Cyclic Tetrapeptides from Auxarthron pseudauxarthron
Journal article   Peer reviewed

Anti-Cryptococcus Phenalenones and Cyclic Tetrapeptides from Auxarthron pseudauxarthron

Yan Li, Qun Yue, Dinith R Jayanetti, Dale C Swenson, Geoffrey A Bartholomeusz, Zhiqiang An, James B Gloer and Gerald F Bills
Journal of natural products (Washington, D.C.), Vol.80(7), pp.2101-2109
07/28/2017
DOI: 10.1021/acs.jnatprod.7b00341
PMCID: PMC5629637
PMID: 28657331
url
http://doi.org/10.1021/acs.jnatprod.7b00341View
Open Access

Abstract

Auxarthrones A-E (1-5), five new phenalenones, and two new naturally occurring cyclic tetrapeptides, auxarthrides A (7) and B (8), were obtained from three different solvent extracts of cultures of the coprophilous fungus Auxarthron pseudauxarthron. Auxarthrones C (3) and E (5) possess an unusual 7a,8-dihydrocyclopenta[a]phenalene-7,9-dione ring system that has not been previously observed in natural products. Formation of 1-5 was found to be dependent on the solvent used for culture extraction. The structures of these new compounds were elucidated primarily by analysis of NMR and MS data. Auxarthrone A (1) was obtained as a mixture of chromatographically inseparable racemic diastereomers (1a and 1b) that cocrystallized, enabling confirmation of their structures by X-ray crystallography. The absolute configurations of 7 and 8 were assigned by analysis of their acid hydrolysates using Marfey's method. Compound 1 displayed moderate antifungal activity against Cryptococcus neoformans and Candida albicans, but did not affect human cancer cell lines.
Antifungal Agents - pharmacology Peptides, Cyclic - isolation & purification Humans Ascomycota - chemistry Crystallography, X-Ray Peptides, Cyclic - pharmacology Antifungal Agents - chemistry Phenalenes - pharmacology Breast Neoplasms - drug therapy Phenalenes - chemistry Peptides, Cyclic - chemistry Cryptococcus neoformans - drug effects Antifungal Agents - isolation & purification Microbial Sensitivity Tests Candida albicans - drug effects Phenalenes - isolation & purification Nuclear Magnetic Resonance, Biomolecular Female Molecular Structure

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