Journal article
Anti-GM1/GD1b M-proteins damage human spinal cord neurons co-cultured with muscle
Journal of the neurological sciences, Vol.120(1), pp.38-45
12/1993
DOI: 10.1016/0022-510X(93)90022-Q
PMID: 8289078
Abstract
gM M-proteins in some motor neuron disease (MND) patients bind immunologically to shared determinants on gangliosides GM1 and GD1b. Since patients with these M-proteins have improved with immunotherapy the antibodies may be important in the pathogenesis of MND. To study how the M-proteins might damage motor neurons, we established co-cultures of human neurons from spinal cord explants and human myotubes. Antibodies from patient but not control serum bound to the cultured neurons. Neurons in co-cultures degenerated after incubation with patient but not control serum. These results demonstrate that anti-GM1 antibodies can bind to and destroy spinal cord neurons that are cultured with muscle. Nerve-muscle co-cultures can serve as a system to examine effects of anti-GM1/GD1b M-proteins on motor neurons.
Details
- Title: Subtitle
- Anti-GM1/GD1b M-proteins damage human spinal cord neurons co-cultured with muscle
- Creators
- Terry Heiman-PattersonThomas KrupaPreston ThompsonEduardo Nobile-OrazioAlbert J TahmoushMichael E Shy
- Resource Type
- Journal article
- Publication Details
- Journal of the neurological sciences, Vol.120(1), pp.38-45
- DOI
- 10.1016/0022-510X(93)90022-Q
- PMID
- 8289078
- ISSN
- 0022-510X
- eISSN
- 1878-5883
- Language
- English
- Date published
- 12/1993
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Stead Family Department of Pediatrics; Iowa Neuroscience Institute
- Record Identifier
- 9984020877902771
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