Journal article
Anti-dihydropyridine Antibodies Exhibit [3H]Nitrendipine Binding Properties Similar to the Membrane Receptor for the 1,4-Dihydropyridine Ca2+ Channel Antagonists
Journal of cardiovascular pharmacology, Vol.9 Suppl 4, pp.S113-S121
1987
DOI: 10.1097/00005344-198700094-00019
Abstract
The [H]nitrendipine binding properties of antidihydropyridine antibodies were characterized and compared with the known [H]nitrendipine binding properties of the membrane receptor for the 1,4-dihydropyridine Ca channel antagonists. Immunization of rabbits with dihydropyridine-protein conjugates resulted in the production of antibodies with high affinity and specificity for the 1,4-dihydropyridine Ca channel antagonists. Anti-dihydropyridine antibodies were found specifically to bind [H]nitrendipine, [H]nimodipine, [H]nisoldipine, and [H]PN200–110 with high affinity, while [H]verapamil, [H]diltiazem, and [H]trifluoperazine were not recognized. [H]Nitrendipine binding to antidihydropyridine antibodies was characterized by a competitive radioimmunoassay. The average dissociation constant of the [H]nitrendipine-antibody complex was 0.06 ± 0.02 nM for an antiserum studied in detail, and ranged from 0.01 to 0.24 nM for all antisera studied. The average dissociation constant determined from rate constants of association (k1 = 2.7 × 10 M s) and dissociation (k = 1.8 × 10s) of [H]nitrendipine from anti-dihydropyridine antibodies immobilized on Protein-A Sepharose was 0.66 nM. Inhibition of [H]nitrendipine binding was specific for the 1,4-dihydropyridine Ca channel modifiers, and the concentrations required for half-maximal inhibition ranged between 0.25 and 0.90 nM; structurally unrelated Ca channel antagonists did not modify [H]nitrendipine binding to the anti-dihydropyridine antibodies. In summary, anti-dihydropyridine antibodies have been shown to have high affinity and specificity for the 1,4-dihydropyridine Ca channel antagonists and to exhibit dihydropyridine binding properties similar to the membrane receptor for the 1,4-dihydropyridine Ca channel antagonists.
Details
- Title: Subtitle
- Anti-dihydropyridine Antibodies Exhibit [3H]Nitrendipine Binding Properties Similar to the Membrane Receptor for the 1,4-Dihydropyridine Ca2+ Channel Antagonists
- Creators
- Kevin Campbell - Department of Physiology and Biophysics, University of Iowa, Iowa City. Iowa, U.S.AAlan SharpSteven Kahl
- Resource Type
- Journal article
- Publication Details
- Journal of cardiovascular pharmacology, Vol.9 Suppl 4, pp.S113-S121
- Publisher
- Lippincott-Raven Publishers
- DOI
- 10.1097/00005344-198700094-00019
- ISSN
- 0160-2446
- eISSN
- 1533-4023
- Language
- English
- Date published
- 1987
- Academic Unit
- Molecular Physiology and Biophysics; Neurology; Iowa Neuroscience Institute
- Record Identifier
- 9984020873002771
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